中国现代中药2018，Vol.20Issue(6)：663-668,6.DOI:10.13313/j.issn.1673-4890.20180131003
利用ISSR分子标记的特征条带鉴别正毛化橘红
Identification of Zhengmao Citri Grandis Exocarpium by ISSR Molecular Markers
摘要
Abstract
Objective:The study aims at establishing an identification method of Zhengmao Exocarpium Citri Grandis by seeking out the characteristic PCR band or bands combination belonging to Zhengmao Citri Grandis Exocarpium based on IS-SR molecular markers. Methods:Proper primers were screened out for PCR,electrophoresis and cluster analysis within 50 ISSR primers.The characteristic PCR band or bands combination belonging to Zhengmao Citri Grandis Exocarpium were sought out and 80 leaf samples of Citri Grandis Exocarpium were validated. Results:The characteristic PCR bands combination of two primers were sought out to identify Zhengmao Citri Grandis Exocarpium and the molecular identification method was established.Conclusion:The established method of characteristic PCR bands combination based on ISSR molecular markers can be used to identify Zhengmao Citri Grandis Exocarpium quickly and accurately.关键词
ISSR分子标记/正毛化橘红/特征条带Key words
ISSR Molecular Markers/Zhengmao Citri Grandis Exocarpium/characteristic bands引用本文复制引用
胡珊,杨志业,邬龙怡,李华,计周正..利用ISSR分子标记的特征条带鉴别正毛化橘红[J].中国现代中药,2018,20(6):663-668,6.基金项目
广东省中医药局科研项目(2017041) （2017041）
