中国现代中药2018，Vol.20Issue(6)：675-679,5.DOI:10.13313/j.issn.1673-4890.20180103004
黄花蒿叶甲醇粗提物对尖孢镰刀菌的抑制作用
Influence of Methanolic Extract of Artemisia annua Leaves on Fusarium oxysporum
摘要
Abstract
Objective:To investigate the antifungal activity of the methanolic extract from Artemisia annua leaves on Fusarium oxysporum.Methods:In vitro experiment was explored by using Oxford Cup method,agar plate surface germination method,acridine orange propidium iodide staining method to determine the effects of the different concentration methanol extract from A.annua leaves on the spore germination rate,spore viability,hypha growth and spore yields of F.oxysporum, which is the main pathogen of Panax notoginseng root-rot diseases.Results:The methanolic extract of A.annua could reduce the spore germination rate and spore activity.Furthermore, it could inhibit the mycelium growth and sporulation of F.oxysporum.With the increasing concentration of the methanol extract from A.annua leaves, the inhibition effect on F.oxysporum became stronger.When the concentration was 25.65 mg·mL－1,compared with the control group,the spore germination rate and spore activity decreased by 13.25% and 12.50%,respectively.The spore yield was also decreased by 90.61% compared with the control group.Conclusion:The methanolic extract from A.annua leaves inhibited the growth of F.oxysporum.Taken together,the present study would lend supports for both ecological control and development of new pesticides of P.notoginseng.关键词
黄花蒿/三七/甲醇粗提物/尖孢镰刀菌/根腐病Key words
Artemisia annua/Panax notoginseng/methanol extract/Fusarium oxysporum/root-rot disease引用本文复制引用
马玉楠,陈传娇,马晓惠,程永现,董鲜,徐福荣..黄花蒿叶甲醇粗提物对尖孢镰刀菌的抑制作用[J].中国现代中药,2018,20(6):675-679,5.基金项目
国家自然科学基金(81660626,81460581) （81660626,81460581）
云南应用基础研究计划-青年项目(2015FD034) （2015FD034）
云南省科学技术厅-云南中医学院应用基础研究联合专项(2017FF116(-014)) （2017FF116(-014）
国家重点研发计划(2017YFD0201402) （2017YFD0201402）
云南省教育厅资助性项目(2017ZZX288) （2017ZZX288）
