中国现代中药2018，Vol.20Issue(6)：691-696,710,7.DOI:10.13313/j.issn.1673-4890.20171111001
桃叶鸦葱黄酮对脑缺血再灌注大鼠的保护作用
Study on Neuroprotective Effect of Total Flavonoids from Scorzonera sinensis on Cerebral Ischemia Reperfusion Injury in Rats
摘要
Abstract
Objective:To study the neuroprotective effect of the total flavonoids from Scorzonera sinensis on cerebral ischemia reperfusion injury in rats.Methods:72 male SD rats were randomly divided into 6 groups:sham-operated group, cerebral ischemia reperfusion model group,positive control group(nimodipine,10.8 mg·kg－1)and three groups of different doses of the total flavonoids from S.sinensis(400,200,100 mg·kg－1,n＝12).The cerebral ischemia-reperfusion model was prepared by middle cerebral artery Occlusion(MCAO)-reperfusion method and reperfusion was performed 2 hours after ischemia. After continuous dosing for 20 d,the neurobehavioral score of rats was graded.The brain water content was determined;the rats'tissue pathological morphological changes were showed by HE staining and the expression of AQP4 in hippocampus of rats was detected with immunohistochemistry.Results:It was showed that model group rats had high neurobehavioral scores and showed obvious symptoms of nerve function defect,while the neurobehavioral scores of high and middle dose total flavonoids and nimodipine groups rats were significantly lower than those of the model group rats'and showed slight nerve function defect (P＜0.05).Compared with model group,high and middle dose of total flavonoids and nimodipine can effectively reduce the cerebral water content in ischemia-reperfusion rats.HE staining showed that the neurons'structure of model group rats had been destroyed,but high and middle dose of total flavonoids and nimodipine could improve this kind of morphology damage. Immunohistochemistry analysis results suggested that the level of AQP4 in the model group rats were significantly higher than those in the sham group(P＜0.01).The level of AQP4 in high and middle dose of total flavonoids and nimodipine group rats were significantly lower than those in the model group(P＜0.01).Conclusion:The cerebral ischemia-reperfusion could cause neuron cells'damage by increasing AQP4,high and middle dose of total flavonoids from S.sinensis and nimodipine could reduce its expression level and improve the nerve defect symptoms.关键词
桃叶鸦葱/脑缺血-再灌注/总黄酮/脑含水量/水通道蛋白4Key words
Scorzonera sinensis/cerebral ischemia reperfusion/total flavonoids/the brain water content/aquaporin 4引用本文复制引用
许丽娟,韵磊,杨辉..桃叶鸦葱黄酮对脑缺血再灌注大鼠的保护作用[J].中国现代中药,2018,20(6):691-696,710,7.基金项目
河北省中医药管理局项目(2015172) （2015172）
