中国现代中药2018，Vol.20Issue(6)：705-710,6.DOI:10.13313/j.issn.1673-4890.20180205007
UPLC-MS/MS测定人参花中5种内源植物激素含量
Determination of Various Endogenous Hormones in Flower Buds of Panax ginseng by UPLC-MS/MS
摘要
Abstract
Objective:To explore the important regulation role of plant endogenous hormones on secondary metabo-lism of Chinese herbal medicine. Methods:Five kinds of endogenous phytohormones(auxin,gibberellin,abscisic acid,jas-monic acid,salicylic acid)in flowers of Panax ginseng were measured by UPLC-MS/MS.Results:The method for determina-tion of 5 endogenous phytohormone content in P.ginseng all has high specificity.The higher content of ABA and SA(plant re-sistance related)was 1 126.17 ± 232.52 ng·g－1and 3 929.43 ± 898.52 ng·g－1respectively;The lower content of IBA and GA3(plant growth related)was 516.70 ± 138.55 ng·g－1and 692.33 ± 228.50 ng·g－1respectively;In jasmonic acid synthesis pathway,the highest content of jasmonate precursor,OPDA,was 47 158.84 + 12 910.55 ng·g－1.JA was not detected and JA-ile was 22.08+6.27 ng·g－1. Conclusion:The study explored the distribution of 5 endogenous phytohormones in ginseng flowers,which laid the foundation for further study on the regulation of endogenous phytohormones on ginsenoside biosynthesis.关键词
UPLC-MS/MS/人参花/植物激素Key words
UPLC-MS/MS/ginseng flower/plant hormone引用本文复制引用
陈康,刘娟,周修腾,纪瑞锋,童宇茹,陈同,袁媛..UPLC-MS/MS测定人参花中5种内源植物激素含量[J].中国现代中药,2018,20(6):705-710,6.基金项目
中央级公益性科研院所基本科研业务费专项资金资助 （）
"十三五"第一批重点领域科研项目"分子生药的研究及应用"(ZZ10-008) （ZZ10-008）
国家自然科学基金(81603235) （81603235）
