中医药学报2018，Vol.46Issue(1)：1-4,4.DOI:10.19664/j.cnki.1002-2392.180001
UPLC-MS/MS MRM模式同时测定参白方中人参皂苷Re、人参皂苷Rg1、人参皂苷Rb1以及Siderin 4种成分含量
Simultaneous Determination of Ginsenoside Re, Ginsenoside Rg1, Ginsenoside Rb1 and Siderin in Shenbai Formula by UPLC-MS/MS MRM
摘要
Abstract
Objective: To establish an UPLC - MS/MS method for the determination of ginsenoside Re, ginsenoside Rgl, siderin and ginsenoside Rbl in Shenbai formula. Methods: Waters ACQUITY UPLCTM HSS T3 column (2.1 mm × 100 mm, 1.8 μm) was adopted; mobile phase; 0.1％ formic acid /methanol and 0.1％ formic acid /water in a gradient mode; electrospray ionization (ESI) and multi reaction ion monitoring (MRM) scanning were applied. Results: Four compounds showed a good linear relationship with good precision, repeatability and stability; the recovery rate was 99.86％ ～ 100.97％, and RSD was 0.91％ ～2.74％. Conclusion: This method is simple, accurate, sensitive and reproducible. It can be used for the content determination of the main constituents in Shenbai formula.关键词
UPLC-MS/MS/参白方/含量测定Key words
UPLC-MS/MS/Shenbai formula/Content determination分类
医药卫生引用本文复制引用
石慧,谢静,宋宏伟,闫广利,韩莹,孟祥才,王喜军..UPLC-MS/MS MRM模式同时测定参白方中人参皂苷Re、人参皂苷Rg1、人参皂苷Rb1以及Siderin 4种成分含量[J].中医药学报,2018,46(1):1-4,4.基金项目
科学技术部"重大新药创制"国家科技重大专项(2015ZX09101043 - 011) （2015ZX09101043 - 011）
科学技术部"重大新药创制"国家科技重大专项(2015ZX09101043 - 005) （2015ZX09101043 - 005）
