中医药学报2018，Vol.46Issue(1)：5-7,3.DOI:10.19664/j.cnki.1002-2392.180002
断体地龙促进创伤愈合活性成分筛选研究
Screening Active Components of Wound Healing from Tail-amputated Earthworm
摘要
Abstract
Objective: To screen out active component of wound healing from the extracts of tail - amputated earthworm. Methods: The extracts were purified by DEAE - Sepharose Fast Flow ion exchange chromatography. The proliferative effects on NIH3T3 were detected by Cell Counting Kit -8 (CCK -8) to filter the most active eluent. Results: The ion exchange chromatography separated the extracts into five fractions after the gradient elution. The results showed that five eluents could promote the proliferation of NIH3T3 fibroblasts, and the cell proliferation rates increased with protein concentration. The proliferation rates of five groups reached the peak at the concentration of 1.5 μg/mL, and the third eluent was regarded as the most active fraction. Conclusion: The third eluent is most effective in promoting effects on proliferation activity of NIH3T3 cells, it may contain potential active ingredients of wound healing, which needs further study.关键词
断体地龙/DEAE-Sepharose Fast Flow/创伤愈合/NIH3T3/CCK-8Key words
Tail-amputated earthworm/DEAE-Sepharose Fast Flow/Wound healing/NIH3T3/CCK-8分类
医药卫生引用本文复制引用
段晓杰,杨雨薇,赓迪,罗世林,王雄飞,丁玉婷,郑竹宏,赵仁云,孙毅坤..断体地龙促进创伤愈合活性成分筛选研究[J].中医药学报,2018,46(1):5-7,3.基金项目
国家自然科学基金面上项目(81373942) （81373942）
