中医药学报2018，Vol.46Issue(1)：11-14,4.DOI:10.19664/j.cnki.1002-2392.180004
薏苡仁谷蛋白源ACE抑制肽的分离纯化
Separation and Purification of ACE Inhibitory Peptides from Glutelin of Coix Seed
摘要
Abstract
To obtain high active ACE (Angiotensin Converting Enzyme) inhibitory peptides from glutelin of Coix seed. Methods: Glutelin of Coix seed was firstly hydrolyzed by pepsin, and the hydrolysate was then separated and purified using the ultrafiltration, ion exchange chromatography and RP - HPLC. Finally, the target peptide fraction was obtained under the guidance of ACE inhibitory activity in vitro. Results: Fraction B2 with higher activity was obtained and the inhibitory rate was 73.41％ ± 1.05％ at the concentration of 0.01 mg/mL. Conclusion: Part components of the glutelin of Coix seed has high potential of anti - hypertensive effect after hydrolysis by pepsin, which provided guidance for further research and development of new peptide drugs from Chinese medicine, to provide new evidence for new application of Coix seed.关键词
薏苡仁/谷蛋白/高血压/分离纯化/ACE抑制肽Key words
Coix seed/Glutelin/Hypertension/Separation and purification/ACE inhibitory peptides分类
医药卫生引用本文复制引用
李开,陈佩瑶,刘露,王灵芝..薏苡仁谷蛋白源ACE抑制肽的分离纯化[J].中医药学报,2018,46(1):11-14,4.基金项目
国家自然科学基金青年基金项目(81102750) （81102750）
北京中医药大学课题(2017 -JYB-XS030) （2017 -JYB-XS030）
