中医药学报2018，Vol.46Issue(1)：15-23,9.DOI:10.19664/j.cnki.1002-2392.180005
砂仁酚类特征成分含量测定及其特征图谱质量表征关联分析研究
Content Determination of Phenols in Fructus Amomi and the Correlation Analysis of Quantity and Quality in Its Specific Chromatogram
摘要
Abstract
Objective: To establish the method for quantitative determination for phenolic characteristic index components and the method correlation analysis of quantity and quality in its specific chromatogram of Fructus Amomi, thus to evaluate the quality of Fructus Amomi effectively and accurately. Methods: HPLC - PDA method was used to determine the contents of five characteristic index component simultaneously in Fructus Amomi, including protocatechuic acid, vanillic acid, catechin, epicatechin, and quercitrin; phenolic specific chromatogram was established in Fructus Amomi, on which basis, the quality of 11 batches of Fructus Amomi was represented according to the number and chemical type of characteristic peaks; the quantitative representation of the 11 batches was established on the contents and peaks area of characteristic index components and flavonoids (represented by catechin) to carry out the correlation analysis. Results: The detected five characteristic index components had good linear correlation and the results of methodological investigation were in accordance with the quantitative determination requirements; batch 5 was taken as the reference substance to evaluate the specific contents, content of the type component, relative contents, peak area, and relative peak area. It showed the overall contents of the characteristic composition of batch 11, 1, 4, 2, 8 and 10 were higher than the reference, and the highest correlation with the reference showed in batch 8, 6, 7, 10, 4 and 3. So the quality of batch 11, 4, 2, 8, 10, 6 and 7 were superior to the others. Conclusion: The established method is simple and accurate, it can be used for the content determination for phenolic characteristic index components in Fructus Amomi. This evaluation mode can be applied into the analysis of overall quality of Fructus Amomi and its effectiveness, which provides a reliable basis for the comprehensive quality evaluation of Fructus Amomi.关键词
砂仁/酚类/特征图谱/质量评价/关联分析Key words
Fructus Amomi/Phenol/Specific chromatogram/Quality evaluation/Correlation analysis分类
医药卫生引用本文复制引用
吕珊,范圆圆,冯鑫,黄玉娟,石任兵,路东波,张元元,孔静,孙仁弟,黄广伟,武秋红,李文霞,李思潼..砂仁酚类特征成分含量测定及其特征图谱质量表征关联分析研究[J].中医药学报,2018,46(1):15-23,9.基金项目
国家科技"十二五"支撑计划项目(2012BAI29B06) （2012BAI29B06）
北京市教委共建科研基地建设项目,北京中医药大学重点学科开放课题(2013-2DXKKF-23) （2013-2DXKKF-23）
