中医药学报2018，Vol.46Issue(1)：48-53,6.DOI:10.19664/j.cnki.1002-2392.180012
熄风胶囊对匹罗卡品IE大鼠血液及脑细胞外液卡马西平药物浓度影响的实验研究
Effect of Xifeng Capsule on the Concentration of Carbamazepine in Blood and Brain Extracellular Fluid in Epileptic Rats
摘要
Abstract
Objective: To observe the effect of Xifeng capsule on the concentration of Carbamazepine (CBZ) in blood and brain extracellular fluid in epileptic rats triggered by aluminium chloride and Pilocapine, to determine whether it could increase the permeability of blood - brain barrier. Methods: Rats were randomly divided into the blank control group(group A, n = 12), the model group (group B, n = 12), CBZ group (group C, n = 12), Xifeng capsule and CBZ group(group D, n = 12 ), Xifeng capsule and 1/2CBZ group (group E, n = 12), high - dose Xifeng capsule group (group F, n = 12), medium - dose Xifeng capsule group(group G, n = 12), and low - dose Xifeng capsule group (group H, n = 12). Epilepsy models were established with Aluminium Choloride and Pilocapine. Every six rats from group C, group D, and group E were tested CBZ concentration in blood and brain extracellular fluid at different time points with microdialysis and HPLC techniques. Results: In comparison with group C, the blood concentration of CBZ in group D was slightly higher than that in group C at each time point, but there was no statistical significance; CBZ concentration in brain extracellular fluid in group D was also increased(30 -90 min, P ＜0.05). CBZ concentrations in blood and brain extracellular fluid were reduced in group E at different time point compared to group D, which were higher than half concentration of CBZ in group D (15 -90 min, P ＜0.05). In terms of CBZ ratio of cerebral and blood it was 0.055: 1 in group C, 0.072: 1 in group D, and 0.073: 1 in group E. Conclusion: Xifeng capsule may increase CBZ concentration in blood and brain extracellular fluid, reinforce the permeablility of blood brain barrier; it can reduce the dose of CBZ, cut down CBZs adverse reaction.关键词
熄风胶囊/卡马西平/血脑屏障/微透析/高效液相色谱法Key words
Xifeng capsule/Carbamazepine/Blood brain barrier/Microdialysis/HPLC分类
医药卫生引用本文复制引用
吴超,李新民,张喜莲,路岩莉,房艳艳,聂坤,刘菁..熄风胶囊对匹罗卡品IE大鼠血液及脑细胞外液卡马西平药物浓度影响的实验研究[J].中医药学报,2018,46(1):48-53,6.基金项目
国家自然科学基金资助项目(81373690) （81373690）
