中医药学报2018，Vol.46Issue(4)：9-13,5.DOI:10.19664/j.cnki.1002-2392.180104
冬连三七组分改善糖尿病动脉粥样硬化兔心肌病理的机理研究
Mechanism Study of CONR Improving Myocardial Injury in DA Rabbits
摘要
Abstract
Objective: To explore the effects of the composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids( CONR) on myocardial injury in rabbits with diabetic atherosclerosis( DA) by reducing RAS system activation and increasing Na+-K+-ATPase activity on the basis of the study that CONR improving protein non-enzymatic glycation( PRG). Methods: DA models were established by alloxan intravenous injection combined with the injury of abdominal aorta intima balloon. The experimental groups were daily given Simvastatin ( 3 mg/kg) and CONR(450 mg/kg, 150 mg/kg, 50 mg/kg) for 10 weeks; the control group was given saline 20 ml. The left ventricu-lar weight( LVW) , cardiac weight( CW) , left ventricular/body mass index( LC/W) , heart body ratio ( C/W) , and the expressions of myocardial angiotensin II( Ang II) , Na+-K+-ATPase, angiotensin converting enzyme( ACE) receptor in cardiac muscle tissue were detected, and myocardial pathology was observed by HE staining method. Results: Com-pared to the model group, CW, LVW, C/W, LC/W, and AngII were significantly reduced(P<0.05), Na+K+-AT-Pase content was significantly increased(P<0.01), and the expression of myocardial ACE receptor was inhibited(P<0.01) in the high-dose CONR group and in the medium-dose CONR group. The improvements of swelling of myocar-dial cells, inflammatory cell infiltration, myocardial interstitial fiber arrangement and other pathological changes were al-so seen in the high-dose CONR group and in the medium-dose CONR group. Conclusion: CONR may improve myo-cardial injury of DA rabbit by inhibiting PRG, reducing RAS system activation and increasing Na+K+-ATPase activity.关键词
冬连三七组分/糖尿病动脉粥样硬化/蛋白非酶糖基化/RAS 系统/Na+-K+-ATP 酶/心肌损伤Key words
Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids/Di-abetic atherosclerosis/Protein non-enzymatic glycation/RAS system/Na+K+-ATPase/Myocardial injury分类
医药卫生引用本文复制引用
靳昭辉,靳冰,高普,宋光熠,刘征堂,相田园..冬连三七组分改善糖尿病动脉粥样硬化兔心肌病理的机理研究[J].中医药学报,2018,46(4):9-13,5.基金项目
国家自然科学基金面上项目(81374026) （81374026）
中国中医科学院名医名家传承项目(05-78) （05-78）
