中医药学报2018，Vol.46Issue(4)：22-27,6.DOI:10.19664/j.cnki.1002-2392.180107
芪杉胶囊对肺癌A549细胞PI3 KAKt信号通路影响的研究
Effect of Qishan Capsule on PI3KAKt Signaling Pathway in Lung Cancer A549 Cells
摘要
Abstract
Objective: To observe the effect of Qishan Capsule on PI3KAKt signaling pathway in lung cancer A549 cells. Methods: The proliferation and apoptosis of A549 cells intervend with medicated serum were detected by MTT method and Annexin V-PE/7-AAD double staining method respectively. Protein and gene expressions related to apoptosis of A549 cells were tested by Western-blot assay and RT-PCR assay. Results: It is found out that different concentration of serum contaning Qishan Capsule had inhibiting effect on the proliferation of A549 cells; the inhibition rate was 24.26% , 16.44% and 10.89% , the higher concentration of the serum was, the more significant inhibitory effect it had on the proliferation of A549 cells. In terms of apoptosis rate, it was significantly increased in serum-Qishan Capsule groups and in cisplatin group, compared to that in the blank control group; the higher concentration of the serum was, the more significant effect it had on apoptosis. In terms of expressions of Caspase -3, Caspase -9, Cytochrome C, AKT, mTOR and PI3K, they were significantly decreased in serum -Qishan Capsule groups and in cisplatin group, compared to those in the blank control group; the higher concentration of the serum was, the more significant decrease was seen on the expressions. Conclusion: Qishan Capsule can inhibit the proliferation and promote apoptosis of A549 cells by regulating the expressions of Caspase-3, Caspase-9, Cytochrome C, AKT, mTOR and PI3K.关键词
肺癌/芪杉胶囊/细胞增殖/细胞凋亡/PI3KAKt信号通路Key words
Lung cancer/Qishan Capsule/Cell proliferation/Apoptosis/PI3KAKt signaling pathway分类
医药卫生引用本文复制引用
隋雨桐,迟文成,姜家康..芪杉胶囊对肺癌A549细胞PI3 KAKt信号通路影响的研究[J].中医药学报,2018,46(4):22-27,6.基金项目
黑龙江省自然科学基金面上项目(H201478) （H201478）
