中医药信息2018，Vol.35Issue(1)：9-11,3.DOI:10.19656/j.cnki.1002-2406.180003
断体地龙成分分离及促进创伤愈合活性研究
Active Fraction of DL3 and Its Would Healing Study
摘要
Abstract
Objective: To investigate the proliferative effect of the extracts from tail - amputated earthworm for three days (DL3) on embryonic fibroblast (NIH3T3 ). Methods: DL3 was separated into four fractions (S1, S2, S3, S4) by salting - out. The proliferation rates of NIH3T3 were detected by Cell Counting Kit - 8(CCK -8) to screen out the active components. Results: It showed that the cell proliferation rates were on the rise with the increasing protein concentration in a certain range. The proliferation rates of four groups reached the peak (113.62％, 108.58％, 117.03％, and 111.26％ ) at the concentration of 6~8 μg/mL, and S3 was the most active fraction. Conclusion: DL3 is effective in promoting proliferation of NIH3T3 cell, which lays the foundation for the further research on wound healing.关键词
断体地龙/盐析/创伤愈合/NIH3T3/CCK-8Key words
Tail-amputated earthworm/Salting-out/Wound healing/NIH3T3/CCK-8分类
医药卫生引用本文复制引用
段晓杰,罗世林,王雄飞,杨雨薇,赓迪,赵仁云,郑竹宏,丁玉婷,孙毅坤..断体地龙成分分离及促进创伤愈合活性研究[J].中医药信息,2018,35(1):9-11,3.基金项目
国家自然科学基金面上项目(No. 81373942) （No. 81373942）
