中医药信息2018，Vol.35Issue(1)：38-42,5.DOI:10.19656/j.cnki.1002-2406.180012
补阳还五汤对局灶性脑缺血大鼠神经干细胞移植后Bag-1表达的影响
Effects of Buyang Huanwu Decoction on Apoptosis Gene Bag -1 after Transplantation of Neural Stem Cells in Rats with Focal Cerebral Ischemia
摘要
Abstract
Objective: To investigate the effects of Buyang Huanwu decoction on apoptosis gene Bag - 1 after transplantation of neural stem cells in rats with focal cerebral ischemia. Methods: 80 SD rats were made into the models of focal cerebral ischemia, assessed by Neurological Function Defect Scale (NFDS), and the rats were randomly divided into five groups after modeling, with 15 rats in each group. After the transplantation of7 d, 14 d, and 28 d, interventions of Buyang Huanwu decoction were applied with respective ultramicro herbal pieces and traditional ones. The neurological deficit scores were determined and expression of apoptosis gene Bag -1 was detected by immunohistochemical staining. Results: For the rats in the simple transplantation and the transplantation group intervened by Chinese medications, their neurological function was obviously improved and the expression of apoptosis gene Bag -1 was raised significantly after the transplantation of 7 d, 14 d, and 28 d, compared to those in the model group(P ＜0.05, P＜0.01); of which, Buyang Huanwu decoction with ultramicro herbal pieces was superior to the traditional ones(P ＜0.05, P ＜0.01). Conclusion: Buyang Huanwu decoction can promote the recovery of neurological function, up - regulate the expression of Bag -1 protein, and the ultramicro herbal pieces were better than the traditional ones.关键词
局灶性脑缺血/神经干细胞/细胞移植/Bag-1/补阳还五汤Key words
Focal cerebral ischemia/Neural stem cell/Cell transplantation/Bag-1/Buyang Huanwu decoction分类
医药卫生引用本文复制引用
廖亮英,蔡光先,周赛男..补阳还五汤对局灶性脑缺血大鼠神经干细胞移植后Bag-1表达的影响[J].中医药信息,2018,35(1):38-42,5.基金项目
国家自然科学基金项目(No. 81302953) （No. 81302953）
湖南省中医药科研计划项目(No. 2014136) （No. 2014136）
湖南省教育厅科研项目(No. 15C1050) （No. 15C1050）
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(No. ZYFT201510) （No. ZYFT201510）
