组织工程与重建外科杂志2018，Vol.14Issue(3)：123-126,4.DOI:10.3969/j.issn.1673-0364.2018.03.002
利用软骨细胞膜片技术体外构建管状软骨组织的实验研究
A Study of Constructing Tubular Tissue Engineered Cartilage by Using Chondrocytes Cell-Sheet Approach
摘要
Abstract
Objective To explore the feasibility of constructing tubular cartilage with a diameter similar to goat tracheal in vitro by large mammalian auricular cartilage,based on improved cell-sheet technology.Methods The chondrocyte cell-sheet was cultured for 1-2 weeks in vitro,and was shaped as tubular cartilage tissue.Samples were collected at 6 weeks.The general appearance and elasticity were observed,and the formation of cartilage was examined.Results After cultured for 6 weeks in vitro,the cell-sheet had intergraded to a white translucent tubular tissue.The tubular tissue had certain biomechanical strength similar to mature cartilage tissues,and the histology analysis displayed continuous cartilage phenotype.Conclusion The improved cartilage cell-sheet technology has a series of advantage including limited defect in donor site,short period in vitro culture,less inflammatory response in vivo and so on.This technology would provide a promising approach for the regeneration of cartilage in vivo.关键词
组织工程软骨/细胞膜片技术/管状软骨Key words
Tissue engineered cartilage/Cell-sheet technology/Tubular cartilage分类
生物科学引用本文复制引用
刘浥,李丹,殷宗琦,周广东,曹谊林..利用软骨细胞膜片技术体外构建管状软骨组织的实验研究[J].组织工程与重建外科杂志,2018,14(3):123-126,4.基金项目
国家自然科学基金(31400830,81270070,81371703,81371701) （31400830,81270070,81371703,81371701）
协和青年基金项目(3332014047) （3332014047）
上海市产学研医项目(15DZ1941600). （15DZ1941600）
