国际医药卫生导报2018，Vol.24Issue(13)：1970-1973,4.DOI:10.3760/cma.j.issn.1007-1245.2018.13.018
PCR-RFLP和PCR-SSCP在食源性感染致病菌检验中的应用
Application of PCR-RFLP and PCR-SSCP in the inspection of foodborne pathogens
摘要
Abstract
Objective To investigate the application of PCR-RFLP and PCR-SSCP in the inspection of foodborne pathogens.Methods 23S rRNA gene of 14 kinds of common foodborne pathogens were set as target genes,universal primers PCR (UP-PCR) was used in amplification,and restriction fragment length polymorphism (RFLP) analysis and single strand conformation polymorphism (SSCP) analysis were used to analyze PCR products digested fragments.Results RFLP for Hpa Ⅱ digestion products 23S rRNA gene fragment analysis showed that RFLP pattern of different species of bacteria had similar performance;SSCP analysis showed that SSCP patterns had greater variability,so could provide an effective basis for the identification of bacteria.Conclusion PCR-SSCP technique can rapidly identify foodbome pathogens,so is worthy of promotion and application in the clinical.关键词
PCR-RFLP/PCR-SSCP/食源性感染致病菌/检验/应用Key words
PCR-RFLP/PCR-SSCP/Foodborne pathogens/Inspection/Application引用本文复制引用
谭炜炜,董进浪,吕建辉..PCR-RFLP和PCR-SSCP在食源性感染致病菌检验中的应用[J].国际医药卫生导报,2018,24(13):1970-1973,4.
