中国防痨杂志2018，Vol.40Issue(4)：348-352,5.DOI:10.3969/j.issn.1000-6621.2018.04.002
免疫组织化学及PCR技术在淋巴结结核病理诊断中的应用价值
Clinical application of immunohistochemistry and PCR technique in pathological diagnosis of tuberculous lymphadenitis
摘要
Abstract
Objective To evaluate the clinical value of immunohistochemistry (IHC) and polymerase chain reaction (PCR) methods in pathological diagnosis of tuberculous lymphadenitis. Methods Paraffin-embedded tissue specimens from 48 patients with tuberculous lymphadenitis who had undergone radical resection (the tuberculosis (TB) group) and 21 cases with other diseases in lymph node (the non-TB group) were collected during Jan 2012 to Jul 2013 in Beijing Chest Hospital, Capital Medical University. One-step IHC using antibody targeting Ag85B,Taqman fluorescent quantitative PCR and acid-fast staining were conducted. Their diagnostic efficiency were analyzed, taking the clinical final diagnosis as golden standard. Results The IHC and PCR showed significantly higher sensitivity than that of acid-fast staining (χ2 =6.27,P=0.012; χ2 =10.84,P=0.001,respectively),by the results of 52.1％ (25/48) and 60.4％ (29/48) versus 27.1％ (13/48). However, there was no significant difference in sensitivity between IHC and PCR (χ2 =0.68,P=0.411). The detection results of IHC,PCR and acid-fast staining in non-TB group were all negative,with a specificity of 100％ (21/21) for each test. The IHC and PCR had higher negative predictive value and accordance rate than acid-fast staining (47.7％ (21/44),52.5％ (21/40),37.5％ (21/56), respectively; 66.7％ (46/69), 72.5％ (50/69),49.3％ (34/69), respectively). Conclusion IHC and PCR methods are simple and have higher sensitivity compared to acid-fast staining method. IHC and PCR showed good clinical application value in pathological diagnosis of tuberculous lymphadenitis.关键词
结核,淋巴结/免疫组织化学/聚合酶链反应/病理学/对比研究Key words
Tuberculosis,lymph node/Immunohistochemistry/Polymerase chain reaction/Pathology/Comparative study引用本文复制引用
董宇杰,张莉,王宇轩,周立娟,曲杨,张晨,张海青,车南颖..免疫组织化学及PCR技术在淋巴结结核病理诊断中的应用价值[J].中国防痨杂志,2018,40(4):348-352,5.基金项目
国家自然科学基金(81572077) （81572077）
首都医科大学附属北京同仁医院头颈部分子病理诊断北京市重点实验室开放研究课题 （）
北京市医院管理局临床医学发展专项(XMLX201506) （XMLX201506）
北京市卫生系统高层次卫生技术人才培养计划(2014-03-082 （）
2014-03-083) （）
首都卫生发展科研专项(2014-4-2161) （2014-4-2161）
北京市医院管理局登峰计划(DFL20151501) （DFL20151501）
北京市科学技术委员会重点项目(D14117005214003) （D14117005214003）
