中国感染控制杂志2018，Vol.17Issue(3)：185-190,6.DOI:10.3969/j.issn.1671-9638.2018.03.001
BBI阻断LPS对肠道上皮细胞间紧密连接蛋白的抑制作用
BBI blocks LPS-mediated inhibitory effect on tight junction protein of intestinal epithelial cells
摘要
Abstract
Objective To evaluate the blocking effect and mechanism of Soybean-derived Bowman-Birk inhibitor (BBI)on LPS-mediated downregulation for tight junction protein(HT-29 cells)in intestinal epithelial cells(IECs). Methods The toxic effect of LPS and BBI on HT-29 cells was detected by CCK8 Kit.HT-29 cells were pretreated by BBI for 6 hours prior to LPS stimulation, the expression of tight junction protein(ZO-1 and Occludin), TLR4, and MyDD8 was detected by the quantitative real-time polymerase chain reaction(PCR)and Western Blot;activation of NF-κB was measured by Western Blot.Results LPS(1 000ng/mL)and BBI(1 000μg/mL)showed no cytotoxicity on HT-29 cells.LPS could significantly upregulate the expression of TLR4 in HT-29 cells, the up-regulation had time-dose effect, and could significantly downregulate the expression of tight junction protein, the down-regulation effect was directly proportional to the concentration of LPS, could activate NF-κB, and had dose effect, effect of LPS on HT-29 cells could be significantly inhibited by BBI.Conclusion By inhibiting the expression of TLR4 and activation of NF-κB in IECs induced by LPS, BBI can significantly block the LPS-mediated inhibitory effect on tight junction protein in intestinal epithelial cells.关键词
胰蛋白酶抑制剂/BBI/肠道上皮细胞/紧密连接蛋白/TLR4/NF-κBKey words
Bowman-Birk inhibitor/BBI/intestinal epithelial cell/tight junction protein/TLR4/NF-κB分类
医药卫生引用本文复制引用
古俊,刘金彪,霍文哲..BBI阻断LPS对肠道上皮细胞间紧密连接蛋白的抑制作用[J].中国感染控制杂志,2018,17(3):185-190,6.基金项目
国家自然科学基金(81271334 （）
81571962) （）
国家传染病防治重大科技专项(2012ZX10004501-001-004) （2012ZX10004501-001-004）
