中国现代医学杂志2018，Vol.28Issue(19)：16-22,7.DOI:10.3969/j.issn.1005-8982.2018.19.004
没食子酸诱导非小细胞肺癌A549细胞凋亡的机制研究
Gallic acid induces apoptosis of non-small cell lung cancer A549 cells via JAK/STAT3 pathway
张庭秀 1马李杰 2范贤明 1肖贞良1
作者信息
- 1. 西南医科大学附属医院 呼吸内科,四川 泸州 646000
- 2. 中国人民解放军成都军区总医院 呼吸内科,四川 成都 610083
- 折叠
摘要
Abstract
Objective To investigate the effect of gallic acid (GA) on proliferation and apoptosis of non-small cell lung cancer A549 cells and the underlying mechanisms. Methods A549 cells were randomly divided into a normal control group and three GA groups. The normal control group was regularly cultured while the GA groups were cultured with different concentrations of GA (12, 20 and 28 μg/ml) for 6-48 h, respectively. The viability of A549 cells was analyzed by MTT assay. Apoptosis induction was detected by flow cytometry. qRT-PCR was used to examine the mRNA expressions of Bax, Bcl-2, JAK1 and STAT3. The protein expressions of Bcl-2, Bax, JAK1, STAT3, p-JAK1 and p-STAT3 were determined by Western blot. Results The viability of the cells in the GA groups was significantly lower than that in the normal control group after 6-48 h culture (P < 0.05), additionally, the percentages of the apoptotic cells in the GA groups were significantly higher than that in the control group after 24 h (P < 0.05). Furthermore, the growth inhibitory effect and the apoptosis induction effect of GA became more and more dramatic with the prolongation of incubation time and increase of GA concentration. It was also found that GA up-regulated Bax mRNA and protein expressions and down-regulated Bcl-2, p-JAK1 and p-STAT3 protein expressions as well as Bcl-2 mRNA level compared with the control group (P < 0.05), however, the mRNA or protein expressions of JAK1 and STAT3 showed no significant differences (P > 0.05). Conclusions GA inhibits viability and induces apoptosis of A549 cells in a dose- and time-dependent manner via blocking the phosphorylation of JAK1 and STAT3 followed by decreased Bcl-2 and increased Bax expressions.关键词
没食子酸/非小细胞肺癌/A549细胞Key words
gallic acid/non-small cell lung cancer/A549 cell分类
医药卫生引用本文复制引用
张庭秀,马李杰,范贤明,肖贞良..没食子酸诱导非小细胞肺癌A549细胞凋亡的机制研究[J].中国现代医学杂志,2018,28(19):16-22,7.
