针灸临床杂志2018，Vol.34Issue(2)：60-64,封3,6.
电针对APP/PS1转基因小鼠海马及血脑屏障Aβ水平的影响
Effect of Electro-acupuncture on Aβ Level of Hippocampus and Blood Brain Barrier in APP/PS1 Transgenic Mice
摘要
Abstract
Objective:To observe the effect of electro-acupuncture on the level of Aβ1-42 in hippocampus and blood brain barrier (BBB), and behavior of APP/PS1 transgenic mice to explore whether electro-acupuncture can improve the ability of Aβ clearance to treat Alzheimer's disease (AD). Methods:24 APP/PS1 transgenic mice (6 months, male) were randomly divided into the electro-acupuncture group and the model group, another 12 C57BL/6 mice were used as the blank control. The electro-acupuncture group was treated with electro-needling GV20 and KI1. After the treatment, Morris water maze was used for behavioral testing to observe the learning and memory abilities in the mice; the expression level of Aβ1-42 in hippocampus was determined by ELISA; the expression of Aβ1-42 in the micro-vascular wall of hippocampus was observed by colloidal gold immuno-electron microscopy. Results:Morris water maze test showed that the escape latency of the model group increased; the times of site crossings, percentage of swimming time and swimming distance were lowered in the model group than those in the blank control group(P ＜0. 01, P ＜0. 05); whereas, the escape latency in the electro-acupuncture group was obviously decreased (P ＜0. 01). The ELISA result showed that the expression of Aβ1-42 in hippocampus was decreased significantly in the electro-acupuncture group compared to that in the model group (P ＜0. 01). The immuno-electron microscopy result showed that the colloidal gold particles in the hippocampal microvascular wall were decreased in the electro-acupuncture group compared to those in the model group. Conclusion:Electro-acupuncture can improve the spatial cognitive ability and reduce Aβ1-42 level of hippocampus in APP/PS1 transgenic mice, which mechanism may be related to the improvement of Aβ clearance ability of BBB.关键词
阿尔茨海默病/电针/β-淀粉样蛋白/海马/血脑屏障/免疫电镜Key words
Alzheimer's disease/Electro-acupuncture/Amyloid beta/Hippocampus/Blood brain barrier/Immuno-electron microscope分类
医药卫生引用本文复制引用
张磊,张学婷,周英奕,裴亚妮,高堂珂,王鑫,薛卫国..电针对APP/PS1转基因小鼠海马及血脑屏障Aβ水平的影响[J].针灸临床杂志,2018,34(2):60-64,封3,6.基金项目
国家自然科学基金项目,编号:81273826. （）
