针灸临床杂志2018，Vol.34Issue(3)：70-73,后插1,5.
早期介入麦粒灸对转基因阿尔茨海默病小鼠脑内APP蛋白表达的研究
Early Intervention of Seed-sized Moxibustion on APP Expression in Transgenic Mice with Alzheimer's Disease
摘要
Abstract
Objective:To explore the therapeutic effect of seed-sized moxibustion on Alzheimer's disease (AD). Methods:The genotyping of transgenic mice were detected by PCR. The 45-day transgenic mice (Tg6799 mice) were randomly divided into the model group(n =9) and the treatment group(n = 8). Nine C57BL/6J female mice of the same age and from the same background were taken as the blank control. The treatment group was applied with seed-sized moxibustion on points of BL15 and BL23, the treatment was once per day for 90 days. Early neuronal injury was observed with Nissl's staining, expression of amyloid precursor protein(APP) in the area of cortex and hippocampus was detected by immunohistochemical method. Results: Nissl's staining showed that there was no obvious neuron loss after the early intervention of seed-sized moxibustion. APP expression in the area of cortex and hippocampus was significantly reduced in the treatment group compared to that in the model group (P ＜ 0. 05). Conclusion:Early intervention of seed-sized moxibustion can reduce the expression of APP in APP/PS1 transgenic mice with AD, thus to restrain the formation of amyloid deposition.关键词
APP/PS1转基因鼠/APP/麦粒灸/阿尔茨海默病Key words
APP/PS1 transgenic mice/APP/Seed-sized moxibustion/Alzheimer's disease分类
医药卫生引用本文复制引用
高灵爱,包烨华,张永生,楚佳梅..早期介入麦粒灸对转基因阿尔茨海默病小鼠脑内APP蛋白表达的研究[J].针灸临床杂志,2018,34(3):70-73,后插1,5.基金项目
浙江省自然科学基金项目,编号:LY13H270002 （）
杭州市科技发展计划项目,编号:2011KJJ16. （）
