中医药信息2018，Vol.35Issue(2)：6-9,4.DOI:10.19656/j.cnki.1002-2406.180040
川牛膝不同组分体内抗凝血活性研究
Active Anticogulants in Vivo of Different Ingredients Extracted from Radix Cyathulae
摘要
Abstract
Objective: To study the anticoagulant activity of the extraction from Radix Cyathulae by different ethanol concentrations. Methods: Solvent reflux extraction and alcohol precipitation were applied with water, 20％ ethanol, and 50％ ethanol, the final concentration was 80％, crude polysaccharides were precipitated. Different concentrations of the crude polysaccharides were administrated to the mice for three continuous days. Afterwards, whole blood coagulation time(CT), bleeding time (BT), prothrombin time(PT), thrombin time(TT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) were tested. Results: The crude polysaccharides extracted by water and by 20％ ethanol could significantly prolong CT, BT, APTT and TT time(P ＜0.05), they could reduce FIB value, but had no obvious effect on PT. However, the crude polysaccharides extracted by 50％ ethanol had no significant effect on the prolongation of CT, BT, PT, APTT, and TT, it did not decrease the FIB value. Conclusion: The crude polysaccharides of Radix Cyathulae extracted by water has significant anticoagulant activity in vivo. Anticoagulant activity of 20％ ethanol extract is secondary to water extract in vivo. 50％ ethanol extract has no significant anticoagulant activity in vivo. Polysaccharides may be the active ingredients of anticoagulation in vivo, they play the anticoagulant role through the intrinsic coagulation pathway and the common coagulation pathway. However, whether polysaccharides are anticoagulant active ingredients needs to be further studied.关键词
川牛膝/粗多糖/抗凝血Key words
Radix Cyathulae/Crude polysaccharides/Anticoagulation分类
医药卫生引用本文复制引用
孟宪群,梁珊珊,赵奕,王秋红,匡海学..川牛膝不同组分体内抗凝血活性研究[J].中医药信息,2018,35(2):6-9,4.基金项目
国家重点基础研究发展计划(973计划)项目(No.2013CB531801) （973计划）
