北京师范大学学报（自然科学版）2018，Vol.54Issue(2)：203-207,5.DOI:10.16360/j.cnki.jbnuns.2018.02.010
浅蛤属(Macridiscus)物种特异性线粒体DNA分子标记及其长度多态性和个体内异质性
Species-specific mitochondrial DNA marker, length polymorphism and heteroplasmy in the clam Macridiscus
摘要
Abstract
In Genus Macridiscus there are three morphologically very similar species: M. melanaegis, M. multifarious and M. semicancellata, the first 2 are sympatric. It is difficult to distinguish these species solely by morphological characters. In this article,genomic DNA was extracted from ethanol preserved foot tissues by aCTAB extraction. A DNA fragment from mitochondrial control region of cytochrome b gene (Cyt b) was used for polymerase chain reaction (PCR) amplification with primers Macr_C01_F16834 and Cytb_Macr_R2. Sequencing of PCR products revealed a 363 bp variable number of tandem repeat (VNTR) close to 3' end of the control region leading to length polymorphism in 44％ heteroplasmic individuals in M. melanaegis. While in the control region of M. semicancellata,two short VNTRs, 14 bp and 17 bp each, were found close to 3' end with length polymorphism between individuals. M. multifarious demonstrated a length conserved control region. These data proved that the three species of Macridiscus could be efficiently and accurately identified via PCR with primer pair Macr_C01_F16834 and Cytb_Macr_R2.关键词
长度多态性/DNA分子标记/线粒体控制区/异质性Key words
length polymorphism/DNA marker/mitochondrial control region/heteroplasmy分类
生物科学引用本文复制引用
施禄也,祁纪伟,高丹丹,韩洁..浅蛤属(Macridiscus)物种特异性线粒体DNA分子标记及其长度多态性和个体内异质性[J].北京师范大学学报（自然科学版）,2018,54(2):203-207,5.基金项目
国家自然科学基金资助项目(210100155) （210100155）
2014年度中央高校基本科研业务费专项资金资助项目 （）
