中国耳鼻咽喉头颈外科2018，Vol.25Issue(1)：17-21,5.DOI:10.16066/j.1672-7002.2018.01.003
下咽鳞状细胞癌CAR-EGFR修饰T细胞构建的初步研究
Preliminary study on the construction of CAR-EGFR modified T cells (EGFR-CART) in hypopharyngeal squamous cell carcinoma
摘要
Abstract
OBJECTIVE Construct and validate the EGFR-CART (Epidermal growth factor receptor-chimeric antigen receptor T) cells targeting the Fadu cell line of hypopharyngeal squamous cell carcinoma, and provide the preclinical basis for the application of CART cell technology in hypopharyngeal squamous cell carcinoma. METHODS EGFR-CART cells and CART-control cells were constructed by lentivirus transfection. The expression of EGFR antibody in CART cells was detected by FACS fluorescence detection, and the expression of EGFR in Fadu cell line and HCT-116 cell line was verified. RESULTS EGFR-CART cells and CART-control cells were successfully constructed, the infection efficiency was about 67％, and there was no significant difference between the two groups in the distribution of cell subsets. HCT-116 is selected as the non target cells of EGFR-CART, Fadu as target cell. CONCLUSION In this study, we successfully prepared and validated the EGFR-CART cells targeting the Fadu cell line of hypopharyngeal squamous cell carcinoma, and laid the foundation for the application of CART technique in the treatment of hypopharyngeal carcinoma.关键词
下咽肿瘤/T淋巴细胞/受体/表皮生长因子/嵌合抗原受体Key words
Hypopharyngeal Neoplasms/T-Lymphocytes/Receptor/ Epidermal Growth Factor/chimeric antigen receptor引用本文复制引用
张洋,丁一鸣,钟琦,陈学军,李平栋,房居高,陈晓红,黄志刚..下咽鳞状细胞癌CAR-EGFR修饰T细胞构建的初步研究[J].中国耳鼻咽喉头颈外科,2018,25(1):17-21,5.基金项目
北京市卫生系统高层卫生技术人才 (2015-3-022) （2015-3-022）
