中国耳鼻咽喉头颈外科2018，Vol.25Issue(1)：27-30,4.DOI:10.16066/j.1672-7002.2018.01.006
水杨酸盐对大鼠听觉中枢超微结构影响的初步研究
Salicylate-induced changes of synaptic ultrastructures in the rat auditory center: a pilot study
摘要
Abstract
OBJECTIVE To observe the changes of synaptic ultrastructures in the rat auditory center after long-term salicylate administration and to elucidate the role of neuroplasticity in some areas of the CNS and its involvement in tinnitus. METHODS The rats were divided into 4 groups: the control group, the acute treatment group, the chronic treatment group, and the recovery group. We investigated ultrastructural alterations in the synapses of inferior colliculus (IC), auditory center (AC) and cerebellum (CRB) by transmission electron microscopy. RESULTS There were more synaptic vesicles (tIC=-4. 61, tAC=-7. 00, P＜0. 01), with greater postsynaptic densities(tIC=-4. 72,P＜0. 01; tAc=-3. 15, P＜0. 05), longer synaptic active zone (tIC=-4. 89, tAC=-3. 48, P＜ 0. 01), and increased synaptic interface curvature (tIC=-2. 32, tAC=-3. 17, P＜0. 05) in the chronic treatment group, as compared with the control group. There were more synaptic vesicles but no other changes in the acute salicylate-treatment group(tIC=-10. 57, tAC=-8. 34, tCRB=-9. 18,P ＜0. 01). CONCLUSION These findings showed that long-term salicylate administration have induced synaptic ultrastructural changes in the IC and AC because of neuroplasticity. These structural changes may result in increased speed and efficacy of chemical synaptic transmission. Alterations to neuroplasticity of the auditory center pathway may lead to tinnitus.关键词
大鼠/模型/动物/耳鸣/水杨酸盐类/神经元可塑性/突触/听觉中枢Key words
Rats/Models/ Animal/Tinnitus/Salicylates/Neuronal Plasticity/Synapses/auditory center引用本文复制引用
易彬,石润杰,吴聪,黄治物,吴皓..水杨酸盐对大鼠听觉中枢超微结构影响的初步研究[J].中国耳鼻咽喉头颈外科,2018,25(1):27-30,4.基金项目
国家自然科学基金资助项目 (81170917、81371091),上海市教委资助项目 (12ZZ103)和上海市耳鼻疾病转化医学重点实验室 (14DZ2260300)联合资助 （81170917、81371091）
