临床与病理杂志2018，Vol.38Issue(3)：472-479,8.DOI:10.3978/j.issn.2095-6959.2018.03.002
乳腺癌EGFL7及β-catenin蛋白检测的临床意义
Clinical significance of EGFL7 and β-catenin protein detection in breast cancer
摘要
Abstract
Objective: To detect and investigate the epidermal growth factor-like domain 7 (EGFL7) and beta chain protein (β-catenin) expression level in tissue and serum in patients with breast cancer and its relationship with metastasis of breast cancer. Methods: EGFL7 and β-catenin protein were detected by immunohistochemistry EnVision two steps method in 93 cases of breast cancer (including 62 cases with lymph node metastasis and 10 cases with distant metastasis) and 54 cases of breast fibroadenoma and adenosis, EGFL7 protein in serum was detected by ELISA double antibody sandwich method in these cases. Results: The protein expression of EGFL7 in breast cancer was higher than that in breast fibroadenoma and adenosis (P＜0.05), the protein expression level of EGFL7 was positive correlation in with lymph node and distant metastasis in breast cancer patients (P＜0.05), but there was no statistical difference between EGFL7 protein expression level in breast cancer with distant metastasis and breast cancer cases with lymph node metastasis (P＞0.05). The β-catenin protein expression in breast cancer tissue was higher than that of benign breast lesions (P＜0.05). The β-catenin protein expression in breast cancer with lymph node and distant metastasis than those without lymph node metastasis in breast cancer tissues (P＜0.05), but there was no significant difference between the β-catenin protein expression level in breast cancer patients with distant metastasis and breast cancer cases with lymph node metastasis (P＞0.05). The EGFL7 protein expression in serum of breast cancer patients with lymph node and distant metastasis was higher than that in non-metastatic breast cancer and breast fibroadenoma and adenosis (P＜0.05), but EGFL7 protein levels were no significant difference in serum of breast cancer patients with distant metastasis compared with the breast cancer with lymph node metastasis (P＞0.05). Conclusion: The detection of EGFL7 and β-catenin protein have a certain clinical reference value for evaluating the risk of metastasis of breast cancer.关键词
乳腺肿瘤/类表皮生长因子域-7/β-链蛋白/免疫组织化学/酶联免疫吸附测定Key words
breast neoplasms/epidermal growth factor-like domain 7/β-catenin/immunohistochemistry/enzyme-linked immunosorbent sorbent assay引用本文复制引用
姜青明,周文文,肖觉..乳腺癌EGFL7及β-catenin蛋白检测的临床意义[J].临床与病理杂志,2018,38(3):472-479,8.基金项目
重庆市卫生和计划生育委员会医学科研重点资助项目(20141019).This work was supported by the Medical Research Funding Project of Chongqing Municipal Health and Family Planning Commission, China (20141019). （20141019）
