中医学报2018，Vol.33Issue(4)：517-520,4.DOI:10.16368/j.issn.1674-8999.2018.04.124
苦参碱对结肠癌SW480细胞凋亡的影响
Effect of Matrine on Apoptosis of Colon Cancer SW480 Cells
摘要
Abstract
Objective: To investigate the effect of matrine on apoptosis of colon cancer SW480 cells induced by Akt signaling pathway and to investigate its mechanism. Methods: Matrine treatment of colon cancer SW480 cells as the experimental group,and blank group as control group,using MTT colorimetric assay to detect the effect on cell proliferation,Hoechst detection of cell apoptosis, flow cytometry detection of cell apoptosis rate,Western blot detection of the effect of matrine on protein expression. Results: After cultured with 24 h, 48 h and 72 h, the SW480 cells in the experimental group showed marked inhibition of growth and increased with the increase of the concentration of drugs and the prolongation of time. The apoptosis rate of SW480 cells in the control group was (4.26± 0.25) ％, significantly lower than the experimental group (9.85± 1.03) ％ and (15.62± 1.98) ％ and(19.69± 2.86) ％ and (26.58± 3.58) ％ and (33.95± 5.26) ％. SW480 cells treated with matrine at different concentrations of 48 h showed that there was significant cell apoptosis,and with the increase of the concentration, the apoptosis rate increased(P ＜ 0.05). Compared with the control group, the content of Bcl-2 in the experimental group decreased, and the content of Bax increased(P ＜ 0.05). In the experimental groups, the content of p-Akt decreased significantly (P ＜ 0.05), and there was no significant change in the total Akt (P ＞ 0.05). Conclusion: Matrine can inhibit the growth of SW480 cells by inhibiting the Akt signaling pathway and induce apoptosis in the cells.关键词
苦参碱/Akt信号通路/结肠癌/SW480细胞/细胞凋亡Key words
matrine/Akt signaling pathway/colon cancer/SW480 cells/apoptosis分类
医药卫生引用本文复制引用
缪冬映..苦参碱对结肠癌SW480细胞凋亡的影响[J].中医学报,2018,33(4):517-520,4.基金项目
国家自然科学基金项目(81060024) （81060024）
