中医学报2018，Vol.33Issue(4)：525-528,4.DOI:10.16368/j.issn.1674-8999.2018.04.126
黄芪多糖抑制人胃癌MKN45、MGC-803细胞生长的作用机制
Inhibitory Effect of Astragalus Polysaccharides on the Growth of Human Gastric Cancer Cell Line MKN45 and MGC-803
摘要
Abstract
Objective: To study the effect of astragalus polysaccharides (APS) on the growth of human gastric cancer cell line MKN45 and MGC-803. Methods: Human gastric cancer cells MKN45 and MGC-803 were placed into culture medium RPMI1640 for subculturing. Cells were divided into control group,5-fluorouracil (5-FU) group and APS group. The control group received 1640 culture medium for culturing; 5-FU group received different concentrations of 5FU for culturing; APS group received different concentrations of APS for culturing. Methyl thiazolyl tetrazolium (MTT) experiment was adopted to test the inhibition of cell growth. Flow cytometry was used to test cell cycle. Fluorescence microscope was used to observe apoptosis. Results: ①After culturing for 24 h,with APS concentration ≥10.0 g·L - 1 and 5-FU concentration ≥0.05 g·L - 1,MKN45 and MGC-803 cells were obviously inhibited and the difference was of statistical significance (P ＜ 0.05); after culturing for 48 h,with APS concentration ≥ 5.0 g·L - 1 and 5-FU concentration ≥0.025 g·L - 1,MKN45 and MGC-803 cells were obviously inhibited and the difference was of statistical significance (P ＜ 0.05). ②After culturing for 48 h,MKN45 and MGC-803 cell cycles of 5-FU group and APS group were all changed, the proportion of cells staying at G0 /G1 phase was obviously higher than that of control group, cells at S phase and G2 /M phase were obviously fewer than those of control group, and the differences were of statistical significance (P ＜ 0.05). ③After culturing for 48 h, apoptosis rates of MKN45 and MGC-803 cells of 5-FU group and APS group were higher than that of control group and the difference was of statistical significance (P ＜ 0.05). Conclusion: APS can inhibit the growth of gastric cancer cells MKN45 and MGC-803 and its possible mechanism of action is to make the cells stay at G0 /G1 phase and induce apoptosis.关键词
人胃癌细胞MKN45/人胃癌细胞MGC-803/肿瘤/黄芪多糖/5-氟尿嘧啶/细胞周期/细胞凋亡Key words
human gastric cancer cell MKN45/human gastric cancer cell MGC-803/tumor/astragalus polysaccharide/5-fluorouracil/cell cycle/cell apoptosis分类
医药卫生引用本文复制引用
桂壮,邵治国,金笛..黄芪多糖抑制人胃癌MKN45、MGC-803细胞生长的作用机制[J].中医学报,2018,33(4):525-528,4.基金项目
国家自然科学基金项目(81302919) （81302919）
