中医学报2018，Vol.33Issue(4)：616-619,4.DOI:10.16368/j.issn.1674-8999.2018.04.148
白藜芦醇对慢性肾衰竭大鼠肾脏组织BMP-7、TGF-β1、Smads信号通路表达的影响
Effect of Resveratrol on the Expression of BMP-7,TGF-β1 and Smads Signaling Pathway in Renal Tissue of Rats with Chronic Renal Failure
摘要
Abstract
Objective: To investigate the effect of resveratrol on the expression of BMP-7, transforming growth factor -β1(TGF-β1)and Smads signaling pathway in renal tissue of rats with chronic renal failure(CRF), and to research the mechanism of delaying CRF progression and antirenal interstitial fibrosis. Methods: 55 rats were selected as the subjects,and chronic renal failure rats were produced by adenine. The rats were divided into blank control group,model group, losartan group, resveratrol low-dose group and resveratrol high-dose group, the changes of serum creatinine(Cr) and blood urea nitrogen(BUN) were observed after treatment. The expression of TGF-β1 mRNA in renal tissue was detected by RT-PCR method, the protein expression of BMP-7,TGF- β1 and Smad6 protein was detected by immunohistochemical method, the expression of BMP-7 and Smad6 protein was detected by Western blot, and the expression of BMP-7,GF-β1 and Smads signal pathway in renal tissue of rat was compared. Results: The levels of Cr and BUN in the high-dose and the low-dose group of resveratrol and the losartan group were significantly lower than that in the model group (P＜0.05); compared to the model group,TGF-β1mRNA expression level in the losartan group,low dose and high dose of resveratrol was significantly decreased,and the difference was statistically significant (P＜0.05); TGF-β1mRNA ex-pression level in the low dose group of resveratrol was lower than the losartan group (P＜0.05).Compared with the model group, BMP-7,Smad6 protein expression in the losartan group,low dose and high dose group of resveratrol were increased,the difference was statistically significant (P＜0.05); compared with the losartan group,BMP-7,Smad6 protein expression improvement in the low dose group of resveratrol was more obvious with statistical significance (P＜0.05); BMP-7 and Smad6 proteins of resveratrol low dose group and high dose group (P＜0.05).Conclusion: Resveratrol can inhibit the expression of TGF-beta 1 by inhibiting the expression of BMP-7 and Smad6 protein,and inhibiting the transduction pathway of TGF-beta 1 signal into the nucleus,so as to al-leviate renal interstitial fibrosis and protect renal function.关键词
白藜芦醇/慢性肾衰竭/信号通路/转化生长因子-β1/BMP-7/Smads/大鼠Key words
resveratrol/chronic renal failure/signal pathway/TGF-β1/BMP-7/Smads/rat分类
医药卫生引用本文复制引用
郑栓..白藜芦醇对慢性肾衰竭大鼠肾脏组织BMP-7、TGF-β1、Smads信号通路表达的影响[J].中医学报,2018,33(4):616-619,4.基金项目
国家自然科学基金项目(81071110) （81071110）
