中医学报2018，Vol.33Issue(4)：620-624,5.DOI:10.16368/j.issn.1674-8999.2018.04.149
四时常服方对氯化镉染毒模型大鼠免疫和抗氧化功能的影响
Effect of Si Shi Chang Fu Prescription on Immune Function and Antioxidant Capacity of Cadmium Infected Rats
摘要
Abstract
Objective: To study the effect of Si Shi Chang Fu prescription on the interleukin-2(IL-2) and superoxidase dismutase(SOD) activity in rats induced by exposure to cadmium, to explore its mechanism. Methods: 60 SPF Wistar rats were randomly divided into blank group,model group,acetylcysteine group,Si Shi Chang Fu prescription low dose group,middle dose group and high dose group. The rats were killed 24 hours after the last administration, and the thoracic spleen index and spleen index were calculated. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) level in serum of rats were measured by colorimetric analysis. The serum levels of interleukin-2 (IL-2) and transforming growth factor- β1(TGF- β1) were measured by ELISA. Results: Weight in the blank group,model group, acetylcysteine group,Si Shi Chang Fu prescription low dose group,middle dose group and high dose group were (193.13±37.48) g,(144.40±11.55) g,(185.10±25.89) g,(162.88±13.11) g,(165.77±15.62) g,(179.20±22.73) g, respectively; indexes of spleen and thymus levels in above groups were (3.96±0.70 /1.69±0.50) mg·g - 1,(1.86±0.43 /0.72±0.33) mg·g - 1,(2.80±0.61 /1.33±0.25) mg·g - 1,(2.49±0.36 /0.83±0.22) mg·g - 1,(2.90±0.53 / 0.87±0.23) mg·g - 1,(3.03±0.72 /1.04±0.39) mg·g - 1, respectively; SOD level in above groups were (72.72±8.10) U·mL - 1,(38.28±4.92) U·mL - 1,(64.87±10.15) U·mL - 1,(56.70±8.94) U·mL - 1,(63.27±7.15) U·mL - 1,(65.54±4.92) U·mL - 1, respectively; MDA level in above groups were(6.26±0.98) nmol·L - 1,(13.36±1.15) nmol·L - 1,(7.86±0.83) nmol·L - 1,(8.09±0.98) nmol·L - 1,(7.19±1.24) nmol·L - 1,(7.00±0.85) nmol·L - 1, respectively; IL-2 level in above groups were (223.61±28.59) ng·L - 1,(130.56±20.18) ng·L - 1,(201.39±23.81) ng·L - 1,(176.67±20.75) ng·L - 1,(187.50±30.16) ng·L - 1,(193.33±38.37) ng ·L - 1, respectively;TGF-β1 level in above groups were (5.50±0.51) μg·L - 1,(11.78±0.83) μg·L - 1,(6.17±0.35) μg·L - 1,(7.93±0.60) μg·L - 1,(7.22±0.78) μg·L - 1,(6.40±0.43) μg·L - 1, respectively. Compared with the blank group, the wight of the model group was significantly reduced (P ＜ 0.05); and the wight of each intervention group was higher than that of the model group (P ＜ 0.05). Compared with the blank group, the spleen and thymus indexes of the model group decreased; and the spleen index increased in each intervention group than that in model group and the thymus index increased in acetylocystcine group than that in model group (P ＜ 0.05). Compared with the blank group, the activity of MDA in the model group was significantly increased(P ＜ 0.05). The level of MDA in each Si Shi Chang Fu prescription intervention group was significantly lower than that in model group (P ＜ 0.05). Compared with the blank group, the level of IL-2 in the model group was significantly lower than that in the blank group, and the level of TGF-β1 was significantly higher than that of blank group(P ＜ 0.05), and the level of IL-2 in each Si Shi Chang Fu prescription intervention group was significantly higher than that in the model group,TGF-β1 was significantly lower than that in the model group(P ＜ 0.05). Conclusion: Si Shi Chang Fu prescriptions has protective effect on oxidative damage and immunity function damage induced by cadmium in rats, its mechanism is achieved by increasing the levels of SOD and IL-2.关键词
四时常服方/氯化镉染毒/氧化损伤/免疫功能/抗氧化能力/超氧化物歧化酶/丙二醛/白细胞介素-2/转化生长因子-β1/胸腺指数/脾脏指数/大鼠Key words
Si Shi Chang Fu prescription/cadmium infected/oxidative damage/immunity function/antioxidant capacity/superoxide dismutase/malondialdehyde/interleukin-2/transforming growth factor-β1/indexes of thymus/spleen index/rat分类
医药卫生引用本文复制引用
罗继红,颜春鲁,安方玉,杨晓蓉,辛阳..四时常服方对氯化镉染毒模型大鼠免疫和抗氧化功能的影响[J].中医学报,2018,33(4):620-624,5.基金项目
甘肃省自然科学基金项目(1508RJZA046) （1508RJZA046）
甘肃中医药大学教学研究与教学改革项目(ZH-201606) （ZH-201606）
