中医药导报2018，Vol.24Issue(8)：68-71,4.
HPLC法同时测定多花蒿中绿原酸类成分的含量
Simultaneous Determination of Chlorogenic Acids in Artemisia Myriantha (多花蒿)by HPLC
荣传兰 1孟勇 2刘丹2
作者信息
- 1. 济南市第五人民医院,山东 济南 250022
- 2. 泰安医学院,山东 泰安 271000
- 折叠
摘要
Abstract
Objective: To establish an HPLC-DAD method for simultaneous determination of 6 types of chlorogenic acids in Artemisia myriantha. Methods: The separation was achieved on a Kromasil 100-5 C18 analytical column (150 mm×4.6 mm, 5 μm) with a gradient elution of 0.1％ acetic acid (A) and acetonitrile (B) at a flow rate of 1.0 mL·min-1. The detection wavelength was 327 nm and the column temperature was 25 ℃. Results: Perfect chromatogram with good separation of 6 phenolic acids including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 4,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 3,5-dicaffeoylquinic acid was obtained within 50 minutes. All the calibration curves showed good linearity between concentration of each compound and peak area. And the values of r were no less than 0.999,7 for all the analytes. The recoveries were 98.06％-98.96％. The developed method was successfully applied for the analysis of 6 chlorogenic acids in Artemisia myriantha obtained from 6 different provinces. Conclusion: The developed method is accurate and reliable, and can be helpful for determination of chlorogenic acids and quality control of Artemisia myriantha.关键词
HPLC-DAD法/同时测定/多花蒿/酚酸类成分Key words
HPLC-DAD/simultaneous determination/Artemisia myriantha/chlorogenic acids分类
医药卫生引用本文复制引用
荣传兰,孟勇,刘丹..HPLC法同时测定多花蒿中绿原酸类成分的含量[J].中医药导报,2018,24(8):68-71,4.
