中医药导报2018，Vol.24Issue(9)：27-30,52,5.
针药联合对荷瘤小鼠HSP70及局部免疫的影响
Effects of Acupuncture Combined with Medicine on HSP70 and Local Immunity in Tumor-Bearing BALB/c Mice
田叶红 1邱晓伟 1易健敏 2姜欣 3李波 3黄金昶4
作者信息
- 1. 延安大学咸阳医院,陕西 咸阳 712000
- 2. 江门市人民医院,广东 江门 529050
- 3. 北京中医药大学,北京 100029
- 4. 北京中医药大学第三附属医院,北京 100013
- 折叠
摘要
Abstract
Objective: To study the effects of peritumoral acupuncture combined with cyclophosphamide(CTX) on HSP70 and local immunity in tumor-bearing mice. Methods: The 4T1 breast tumor-bearing mice were randomly divided into model group, drug group, acupuncture group and group of acupuncture combined drug. The model group was not intervened; The drug group received intraperitoneal injection with CTX (50 mg/kg) every other day; The acupuncture group received peritumoral acupuncture treatment every day; The group of acupuncture combined drug received peritumoral acupuncture and intraperitoneal injection with CTX. After 2 weeks of experimental intervention, inhibition rate was observed. HE staining and IPP6.0 were used to observe the expression of HSP70. Flow cytometry was used to detect the expression of PD-L1 and CD4+CD25+Treg in tumor tissue. Results: Acupuncture or CTX could inhibit tumor growth and reduce the expression of HSP70. Acupuncture combined with CTX showed synergistic effect on tumor growth and the expression of HSP70. CTX can reduce the expression of CD4+CD25+Treg cells and increase the positive rate of PD-L1, but acupuncture or acupuncture combined showed no significant effect. Conclusion: Acupuncture combined with CTX can inhibit the expression of HSP70 in tumor microenvironment, but it shows no obvious effect on the expression of PD-L1 and Treg cells.关键词
瘤周围刺/环磷酰胺/4T1/HSP70/Treg细胞/PD-L1Key words
peritumoral acupuncture/Cyclophosphamide/4T1/HSP70/treg cell/PD-L1分类
医药卫生引用本文复制引用
田叶红,邱晓伟,易健敏,姜欣,李波,黄金昶..针药联合对荷瘤小鼠HSP70及局部免疫的影响[J].中医药导报,2018,24(9):27-30,52,5.
