中医药导报2018，Vol.24Issue(10)：14-18,5.
基于多指标综合检测优选藏药砂生槐子总生物碱提取工艺及其抗肝纤维化机制研究
Optimization of Extraction Technology of Total Alkaloids from Sophora Moorcroftiana (砂生槐子) Based on Multi Index Comprehensive Detection and Its Mechanism of Anti-hepatic Fibrosis
摘要
Abstract
Objective: To investigate the optimum extraction technology of total alkaloids from sophora moorcroftiana and evaluate its anti-hepatic fibrosis effect. Methods: The content of oxymatrine, sophocarpine, sophoridine and matrine in sophora moorcroftiana were detected by HPLC, and the best extraction process was investigated by orthogonal test of L9 (34). The experimental liver fibrosis was induced by CCl4 in rats. The indexes of ALT, AST, HA, TGF-β and Hyp in blank group, model group, colchicine group, high, middle and low Sophora moorcroftiana dose groups were detected in the 3, 6, 9 weeks, and serum contents of miR-181 b, miR-16, miR-200 a and miR-126 were determined. Results: Sophora Moorcroftiana with particle size of 60 mesh were heated2 times in 15 volume of 70％ ethanol, and the contents of 4 alkaloids were 80. 10, 3. 92, 9. 26 and 19. 60 mg/g, respectively. In the experimental period, all indexes of the high, middle and low Sophora moorcroftiana dose groups were lower than the model group with a concentration dependent manner, which showed a significant inhibitory effect of the total alkaloid on liver fibrosis. The expression of miR-181 b in high dose group decreased significantly, while the expression of miR-16, miR-200 a and miR-126 increased significantly. Conclusion: The optimum extraction process can be obtained through the optimization of orthogonal test. The total alkaloid has a certain therapeutic effect on CCl4 induced experimental hepatic fibrosis in rats and the mechanism may be related to its action on hepatic stellate cells.关键词
砂生槐子/总生物碱/提取工艺/抗肝纤维化/大鼠Key words
sophora moorcroftiana/total alkaloids/extraction process/anti-hepatic fibrosis/rats分类
医药卫生引用本文复制引用
闵慧,胡春晖,胡斌,李琼阁,王静,薛小荣..基于多指标综合检测优选藏药砂生槐子总生物碱提取工艺及其抗肝纤维化机制研究[J].中医药导报,2018,24(10):14-18,5.基金项目
青海省科技厅项目(2014-ZJ-716) （2014-ZJ-716）
教育部"春晖计划"(Z2015077) （Z2015077）
