中医药导报2018，Vol.24Issue(11)：36-39,44,5.
脊柱推拿对脑瘫幼鼠学习记忆的影响及机制研究
Influence and Mechanism of Spinal Tuina on Learning and Memory in Juvenile Rat with Cerebral Palsy
摘要
Abstract
Objectives: To observe the effect of Spinal Tuina on the learning and memory in the hippocampus of cerebral palsy (CP) juvenile rats, and explore the mechanism. Methods: A total of 40 male juvenile rats were randomly divided into blank control group, control+Tuina group, model group and model+Tuina group, with10 rats in each. The model group and the model+Tuina group were operated to establish CP model in postnatal3 days (P3). After P5, the control and Tuina group and model and Tuina group were given spinal Tuina for 28 d. Morris water maze (MWM) and Step-down test (ST) were commenced respectively to observe learning and memory abilities. The levels of 5-hydroxytryptamine (5-HT), norepinephrine (NE), adrenaline (E) and dopamine (DA) in hippocampus tissue were detected by high performance liquid chromatography. Results: The model group and the model+Tuina group showed longer incubation of finding the platform than the blank control group in the MWM, and the control +Tuina group showed higher number of crossing the platform than blank control group (P<0. 05). The model group showed short incubation period than the blank control group as well as more times of jumping down the platform in the ST (P<0. 05). The model group showed lower content of NE and 5-HT hippocampus tissue than the blank control group (P<0. 05). The ontrol+Tuina and model+Tuina group showed higher content of adrenaline than the blank control group (P<0. 05). The control+Tuina and model+Tuina group showed higher content of adrenaline than the model group (P<0. 05). The model group showed higher content of DA than the blank control group (P<0. 05). Conclusion: Spinal Tuina can improve the learning and memory ability of juvenile rats with cerebral palsy. It may be related to the regulation of monoamine neurotransmitters such as 5-HT, NE, E and DA in the hippocampus.关键词
脑瘫/脊柱推拿/学习记忆/幼鼠/迷宫试验/跳台实验/高效液相色谱法Key words
cerebral palsy/spinal Tuina/learning and memory/juvenile rat/maze test/step-down test/high performance liquid chromatography分类
医药卫生引用本文复制引用
何琪,张骞,邰先桃,袁恺,张星贺,朱博文,杨叶娇,李炳..脊柱推拿对脑瘫幼鼠学习记忆的影响及机制研究[J].中医药导报,2018,24(11):36-39,44,5.基金项目
国家自然科学基金项目(No.81460748) （No.81460748）
