基础医学与临床2018，Vol.38Issue(2)：174-179,6.
长链非编码RNA EVADR促进人结直肠癌细胞系增殖及迁移
IncRNA EVADR promotes proliferation and migration of human colorectal cancer cell lines
摘要
Abstract
Objective To explore the effect and mechanism of IncRNA EVADR on the proliferation and migration in human colorectal cancer cell lines HCT116 and LOVO. Methods HCT116 and LOVO cell lines were transfected with IncRNA EVADR by overexpressing lentivirus system. CCK8 assay was performed to measure the growth of HCT116 and LOVO cells after overexpression of EVADR. Transwell migration was performed to determine if EVADR promote HCT116 and LOVO cells migration. Finally, the expression of Ecadherin and transcription factor Snail, Slug, ZEB1 and ZEB2 were detected by Western blot and realtime quantitative PCR respectively. Results We successfully established colorectal cancer cells strains HCT116, LOVO which can stably overexpress IncRNA EVADR and the capacity of proliferation and migration in overexpression group was significantly improved (P＜0.05). The expression of Ecadherin was decreased while mesenchymal markers Snail, Slug, ZEB1 and ZEB2 were increased in EVADR overexpression HCT116 and LOVO cells. Conclusions Overexpression of IncRNA EVADR in HCT116 and LOVO cells can significantly promote the proliferation and migration of HCT116 and LOVO cells which may play an important role in regulating epithelial-mesenchymal transition in colorectal cancer cells.关键词
结直肠癌/IncRNA EVADR/增殖/迁移Key words
colorectal cancer/IncRNA EVADR/proliferation/migration分类
医药卫生引用本文复制引用
韩丹,童亚楠,贾银平,唐梦灵,曹刘素,张竹君,潘渠..长链非编码RNA EVADR促进人结直肠癌细胞系增殖及迁移[J].基础医学与临床,2018,38(2):174-179,6.基金项目
国家自然科学基金(31170007) （31170007）
