基础医学与临床2018，Vol.38Issue(2)：205-212,8.
CD11c+DCs促K14-VEGF转基因小鼠银屑病样发病
CD11c+DCs promote the occurrence and development of psoriasis-like morbidity in K14-VEGF transgenic mice
摘要
Abstract
Objective To investigate the auxoaction and mechanism of CDllc+DCs on psoriasis. Methods CDllc+ DCs in the psoriasis-like lesions were confirmed by immunofluorescence double staining experiments. The CD1lc+ DCs were subcutaneously injected into the neck skin of transgenic mice without disease. The clinical features were observed and assessed with PASI score every day. The ear and back skin were checked by HE stained. At the same time, the T lymphocyte and DCs of bone marrow, spleen, submandibular lymph nodes and blood were analyzed by flow cytometry, and the T lymphocyte in the skin lesions were analyzed by immunofluorescence. The heart, liver, spleen, lungs and kindey were HE stained. Results In the psoriasis-like lesions, about 90％ CDllc+ cells expressed CDllc. HE test showed that the thickness of the skin layer was significant different between the experimental group (ear: 29±4 μm; back: 25±3 μm) and the control group(ear: 11±2 μm; back: 9±1 μm) (P＜0.01). CD3 + CD4+ T lymphocyte cells in the blood of the experimental mice (17.87％) were decreased significantly (P＜0.01) compared with the control group mice (31.77％). The numbers of Thl and Th17 cells from bone marrow, spleen, submaxillary lymph nodes and blood, as the same as CD1lc+DCs from bone marrow and submaxillary lymph nodes, were decreased in the experimental mice compared with the control group mice (P＜0.01). The number of CD4﹢T cells, CD8+T cells, IL-17a+cells and IFN-γ+cells in the skin lesions from the experimental group all were higher than that from the control group (P＜0.01). And the vessels in the lesions of the experimental group were higher than that in the control group (P＜0.01). Conclusions CD1 lc+CD1 lc+DCs may play an important role in the occurrence and development of psoriasis by increasing the T lymphocyte and the blood vessel in the skins of K14-VEGF transgenic mice.关键词
CDllc+DCS/K14-VEGF转基因小鼠/银屑病/免疫调节Key words
CDllc+DCs/K14-VEGF transgenic mice/psoriasis/immune regulation分类
医药卫生引用本文复制引用
张怡,周彪,郭政宏,龙虎,严亨秀..CD11c+DCs促K14-VEGF转基因小鼠银屑病样发病[J].基础医学与临床,2018,38(2):205-212,8.基金项目
西南民族大学省级大学生创新创业训练计划(s201710656049) （s201710656049）
西南民族大学校级教改重大培育项目(2017ZDPY01) （2017ZDPY01）
四川省教改项目"兽 医 专业学位研究生教育实践基地建设"(20140108) （20140108）
