临床输血与检验2018，Vol.20Issue(1)：7-9,3.DOI:10.3969/j.issn.1671-2587.2018.01.003
微柱凝胶免疫实验检测患者血清中抗肿瘤药物类抗体的临床应用
Micro-column Gel Immunoassay forDetection ofAntibodies against Anti-tumor Drugs
摘要
Abstract
Objective To detect the serumantibodies against oncotherapy agentsand provide clinical datafor safety of transfusion and chemotherapy. Methods A total of 309 serum samples were randomly selected from patients with chemotherapy and antibodies were detected. Antibodies tocapecitabine and trastuzumab were detected in 157 and 152 patients, respectively. The patient's seraweremixed with O-type erythrocytes coated with the corresponding drugs, and the micro-column gel indirect anti-human globulin method wasperformed to detect the antibodies against the two agents. Result Among 309 serum samples, eight were found to bepositive, with ageneral positive rate of2.5％, including 3 (1.9％) of Trastuzumab and 5 (3％) of capecitabin. Conclusion Detection of the antibodies against anti-tumor chemicals would be necessary toprevent from immune hemolysis and other adverse reactions post-transfusion.关键词
抗肿瘤药物抗体/微柱凝胶法/免疫性溶血Key words
Antibody against cancer therapeutic agent/Micro-column gel assay/Immune hemolysis分类
医药卫生引用本文复制引用
刘娟,陈柄澔,卢江敏,郑飞天,候杜娟,孟方园,李翠莹..微柱凝胶免疫实验检测患者血清中抗肿瘤药物类抗体的临床应用[J].临床输血与检验,2018,20(1):7-9,3.基金项目
本课题受军区十二五重点项目(No.B14009)资助 （No.B14009）
