临床输血与检验2018，Vol.20Issue(1)：20-23,4.DOI:10.3969/j.issn.1671-2587.2018.01.008
亚甲蓝病毒灭活血浆中人细小病毒B19抗原、抗体的检测与分析
Detection and Analysis of Human Parvovirus B19 Antigens and Antibodies in Methylene Blue Inactivated Virus-free Plasma
摘要
Abstract
Objective To detect and analyze the human parvovirus B19 antigens and antibodies in methylene blue inactivated virus-free plasma to evaluate the safety of blood transfusion. Method 1 056 bags of plasma inactivated with methylene blue were randomly selected and prepared from February to April, 2016, for detection of the human parvovirus B19 antigens and antibodies using ELISA. Samples of the positive HPV B19 antibodies were grouped for statistical analyses according to gender, age, and blood groups. Results Parvovirus B19 virus antigens were negative in all 1056 bags of methylene blue inactivated virus-free plasma, whereas IgG and IgM antibodies were positive with the overall prevalence of20.5％ (216/1056), with 19.5％ (206/1056) IgG positive rate being, and 4.9％ (52/1056) IgM positive rate. The HPV B19 antibody positive rate were found to be different among different blood donors: B19 positive rate increased with age, antibodies to B19 in blood group A and AB was higher than in groups B and O.No statistically significant difference was noted in gender. Conclusion Although the risk of Yichang residents at human parvovirus B19 infection from blood transfusion is not high, detection of HPV B19 before blood transfusion is significant for some recipients such as immunocompromised patients.关键词
亚甲蓝病毒灭活血浆/人细小病毒B19/血液安全Key words
Methylene blue inactivated virus-free plasma/Human parvovirus B19/Blood safety分类
医药卫生引用本文复制引用
李鹏,郭毅..亚甲蓝病毒灭活血浆中人细小病毒B19抗原、抗体的检测与分析[J].临床输血与检验,2018,20(1):20-23,4.基金项目
本课题受宜昌市医疗卫生科研项目(No.A14301-45)资助 （No.A14301-45）
