农业生物技术学报2018，Vol.26Issue(7)：1143-1154,12.DOI:10.3969/j.issn.1674-7968.2018.07.006
干旱胁迫下花生差异表达转录因子家族分析
Differential Expression of Transcription Factor Families in Peanut (Arachis hypogaea) Under Drought Stress
摘要
Abstract
Peanut(Arachis hypogaea) is an important crop,but it is badly affected by drought stress.The draft peanut genome sequence released in 2016 can be used to help understand the molecular mechanisms of drought tolerance in this species.Transcription factors (TFs) play central roles in defense responses in peanut.In this study,the differential expression of TFs in response to drought stress in the drought-resistant peanut cultivar Jll by RNA-seq was analyzed.Pooled samples obtained at 6,12,18,24,and 48 h were compared with control samples at 0 h.In total,this study identified 236 differentially expressed TFs based on the transcriptome data;142 were up-regulated and 94 were down-regulated.Among the differentially expressed TFs,members of the MYB and bHLH families were the most abundant.In up-regulated TFs group,the fold change range was from 2.034 1 to 43.968 3,and 69 TFs was＞10.In down-regulated TFs group,the fold change range was from-2.1031 to-11.6103,and 58 TFs was ＜-4.In this study,25 MYB genes were induced and 17 were down-regulated;17 bHLH genes were up-regulated and 14 were down-regulated;all NAC TFs was used to were up-regulated.In order to validate the gene expression data obtained by RNA-seq,qRT-PCR was used determine the expression levels of 14TF genes.Although the data obtained from qRT-PCR and RNA-seq were different,the trends in the expression levels determined by qRT-PCR were consistent with those obtained by RNA-Seq,thereby confirming the accuracy of the RNA-seq results.The results confirm the idea that drought resistance in peanut was largely dependent upon multiple TF families.The results of this study will provide a useful resource for further molecular research in this species.关键词
转录因子/干旱胁迫/花生/转录组测序Key words
Transcription factor/Drought stress/Peanut/RNA-seq分类
农业科技引用本文复制引用
赵小波,闫彩霞,张浩,王娟,李春娟,谢宏峰,单世华..干旱胁迫下花生差异表达转录因子家族分析[J].农业生物技术学报,2018,26(7):1143-1154,12.基金项目
山东省自然科学基金(No.ZR2016CP03)、国家国际合作专项(No.2015DFA31190)、山东省农业科学院青年基金(No.2016-YQN16)、山东省农业产业技术体系(No.SDAIT-04-02)、山东省农业良种工程(No.20141z010)和泰山学者特聘专家(ts201712080) （No.ZR2016CP03）
