山东中医杂志2018，Vol.37Issue(5)：409-411,3.DOI:10.16295/j.cnki.0257-358x.2018.05.018
针刺夹脊穴对DOP模型大鼠骨钙素、破骨细胞CK表达影响的实验研究
Effects on the Expression of Osteocalcin and Osteoclast Cathepsin K in Diabetic Osteoporosis Rats by Needling Jiaji points(EX-B2)
摘要
Abstract
Objective:To investigate effect on the expression of osteocalcin and osteoclast cathepsin K (CK) in rats model of diabetic osteoporosis (DOP) by needling Jiaji points (EX-B2). Methods:There were 50 SD male rats, in which 10 rats were used as the control, 40 rats were given intraperitoneal injection of streptozotocin(STZ) combined with high fat and high glucose diet to make DOP model. The qualified DOP model rats were randomly divided into acupuncture group, positive drug group and model group, and were given acupuncture at Jiaji points (EX-B2), calcium carbonate and alfacalcidol, saline gavage, respectively. After 6 weeks of intervention, bone mineral density (BMD), bone histomorphometry, serum osteocalcin (OC), and osteoclast CK were detected. Results:After 6 weeks of intervention, compared with the DOP models, acupuncture could increase the BMD obviously with statistical difference(P＜0.01), increase expression of serum OC, suppress expression of osteoclast CK of the DOP rats, but there was no statistical difference compared with the positive drug group (P＞0.05). Conclusion:Acupuncture on Jiaji points (EX-B2) could increase expression of OC and suppress expression of osteoclast CK to regulate bone metabolism, and hence it is effect for relieving bone loss in DOP rats.关键词
糖尿病性骨质疏松症/夹脊穴/骨代谢/实验研究Key words
diabetic osteoporosis/Jiaji points(EX-B2)/bone metabolism/experimental study分类
医药卫生引用本文复制引用
程斌,张莉莉,魏峰,于慧娟,张昕..针刺夹脊穴对DOP模型大鼠骨钙素、破骨细胞CK表达影响的实验研究[J].山东中医杂志,2018,37(5):409-411,3.基金项目
国家自然科学基金青年项目(编号:81202650) （编号:81202650）
山东省优秀中青年科学家科研奖励基金项目(编号:BS2014YY027) （编号:BS2014YY027）
山东省中医药科技发展计划项目(编号:2015-111,2011-102) （编号:2015-111,2011-102）
山东省医药卫生科技发展计划项目(编号:2013WS0129) （编号:2013WS0129）
