中国农业大学学报2018，Vol.23Issue(5)：67-73,7.DOI:10.11841/j.issn.1007-4333.2018.05.08
哺乳人溶菌酶转基因乳汁提高仔鼠肠道健康水平
Consumption of human lysozyme transgenic milk improved intestinal health of mouse pups
摘要
Abstract
The aim of this study was to investigate the effect of human lysozyme transgenic milk on intestinal health. The mouse pups were nursed randomly by transgenic mother mice (PC group) and non-transgenic mother mice (NC group), and the experiments as follows were performed on day 3,11,21,30 (D3,D11,D21 and D30): the number of intestinal bacterial flora was counted,the fecal morphology of mouse pups in different groups was observed, and the mice were induced to diarrhea models by injection of Escherichia coli. The results showed that: 1) The total bacteria in PC group was significantly lower than that in NC group at D11 and D21 (P ＜ 0.05); The quantity of beneficial bacteria Lactobacillus spp. in PC group was significantly higher than that in NC group at D21 (P ＜ 0.05),and the Bifidobacterium spp. was also significantly higher than that in NC group at D11 and D30 (P＜0.05); The quantity of harmful bacteria E. coli in PC group was significantly lower than that in NC group at D21 (P＜0.05),the Streptococcus was very significantly lower than that in NC group at D11 (P＜0.01) and significantly lower than that in NC group at D21 (P＜0.05), the Enterococcus was very significantly lower than that in NC group at D11 (P＜0.01), and the Clostridium was significantly lower than that in NC group at D11 (P ＜ 0.05).2) The lysozyme activities of small intestine contents were 1803土93 U/μg in PC group, while was only 73±23 U/μg in NC group; the semisolid stools were formed in the proximal/middle of colonin in PC group(9/10), whereas in the middle/end of large intestine in NC group (8/10).3) The mortality rate of diarrhea mice model in NC group was 60％,while in PC group was 0. Inconclusion, recombinant human lysozyme of the transgenic mice kept high activity in the small intestine of mouse pups,which significantly increased the number of beneficial bacteria,reduced the number of harmful bacteria in the intestine,promoted semisolid stool formation and provided protection for the mouse diarrhea model.关键词
重组人溶菌酶/转基因小鼠/哺乳仔鼠/肠道菌群/小鼠腹泻模型Key words
recombinant human lysozyme/transgenic mice/mouse pups/intestinal flora/mouse diarrhea model分类
生物科学引用本文复制引用
杨雪珍,鲁丹,商圣哲,王宇航,王晔,林树茂,张辉华,刘燊..哺乳人溶菌酶转基因乳汁提高仔鼠肠道健康水平[J].中国农业大学学报,2018,23(5):67-73,7.基金项目
广东省科技计划公益研究与能力建设专项(2015A020209195) （2015A020209195）
佛山市科技创新专项市院合作项目(2014HK100314) （2014HK100314）
