中国农业大学学报2018，Vol.23Issue(5)：74-85,12.DOI:10.11841/j.issn.1007-4333.2018.05.09
苜蓿镰孢菌根腐病病原致病性和毒素化学型测定
Detection of pathogenicity and mycotoxin chemotypes of Fusarium causing alfalfa root rot
摘要
Abstract
Alfalfa(Medicago sativa)is widely cultivated in the world.Alfalfa root rot has become one of the most important factors resulting in quality decline and yield losses of alfalfa.To explore the difference in pathogenicity and the pathogenic mechanism of Fusarium causing alfalfa root rot,150 Fusarium strains isolated from the samples of alfalfa root rot collected from Huanghua and Xuanhua District in Hebei Province,China,among which 72 strains were isolated in 2015 and 78 strains were isolated in 2016,were used in this study.Pathogenicity determination of all Fusarium strains was conducted using plate method.The DON(deoxynivalenol)and NIV(nivalenol)mycotoxins producing potentialities of the strains were investigated.And bioactivity tests of crude mycotoxins derived from strain QD3-2(F.oxysporum), which was identified as NIV chemotype,were performed.The results showed that all the isolated Fusarium strains induced pathogenicity on germinated alfalfa seeds,but there were differences among the pathogenic intensities.Most Fusarium strains exhibited strong pathogenicity except F.tricinctum strains and most F.equiseti strains.Strain KD3(F.oxysporum),strain QD3-2(F.oxysporum),strain N6-1(F.equiseti),strain N9-2(F.acuminatum),strain NT1-1(F.acuminatum)and strain QD10-1(F.acuminatum)displayed potential to produce NIV,and there were no strains showed DON producing potentiality.After fermentation,crude mycotoxins were extracted from strain QD3-2 using organic solvents.The bioactivity tests demonstrated that the crude mycotoxins had strong inhibitory effects on the germination of alfalfa seeds.This study would provide theoretical references for further studies on the pathogenic mechanism of pathogens causing alfalfa root rot,efficient management of alfalfa root rot and alfalfa production safety.关键词
苜蓿根腐病/镰孢菌/致病性/毒素化学型/粗毒素Key words
alfalfa root rot/Fusarium/pathogenicity/mycotoxin chemotype/crude mycotoxin分类
农业科技引用本文复制引用
孔前前,秦丰,张雨竹,马占鸿,刘志龙,王海光..苜蓿镰孢菌根腐病病原致病性和毒素化学型测定[J].中国农业大学学报,2018,23(5):74-85,12.基金项目
公益性行业(农业)科研专项经费项目(201303057) （农业）
