中国农业大学学报2018，Vol.23Issue(5)：86-92,7.DOI:10.11841/j.issn.1007-4333.2018.05.10
高产脂肪酶菌株的筛选鉴定
Screening of high lipase-producing strains
摘要
Abstract
Lipase-producing strains isolated from soil were screened by the color-changing circle method and transparent circle method.These strains were identified according to their morphology,Gram staining and 16SrDNA sequence analysis.A total of 51 high lipase-producing strains were isolated on Rhodamine B screening plates by using HC index.Compared the HC of the strains screened by olive oil screening plates,L-17,the strain of highest HC 2.27, was identified as target strain.The enzyme activity of L-17 was determined by transparent circle method,and its HC was 3.07 which proved the high enzyme activity of L-17.The L-17 strain was identified as Burkholderia cepacia with high lipase yield,which had potential research and development value.关键词
脂肪酶/筛选/鉴定/洋葱伯克霍尔德菌Key words
lipase/screening/identification/Burkholderia cepacia分类
轻工纺织引用本文复制引用
谢清,王瑶,陈尚武,李平兰..高产脂肪酶菌株的筛选鉴定[J].中国农业大学学报,2018,23(5):86-92,7.基金项目
现代农业产业体系北京市创新团队建设-鲟鱼、鲑鳟鱼(SCGWZJ201711) （SCGWZJ201711）
