中国农业大学学报2018，Vol.23Issue(6)：70-76,7.DOI:10.11841/j.issn.1007-4333.2018.06.08
基于Y染色体ZFY-10标记多态性探究柴达木黄牛父系支系组成和起源
Explore the patrilineal branches and origin of Qaidam cattle based on the polymorphism of Y chromosome ZFY-10 marker
摘要
Abstract
To explore the paternal genetic structure of Qaidam cattle (Bos taurus), the genetic diversity and background were investigated at molecular level. By using 8 male Dabieshan zebu as experimental control, the polymorphism of Y chromosome ZFY-10 marker in 106Qaidam cattle was analyzed based on PCR amplification and direct sequencing. The results showed that:1)The variations identified by ZFY-10 marker could be used to distinguish Bos taurus haplogroup Y1 and Y2, and Bos indicus haplogroup Y3;2)The polymorphism of GT insert/deletion on ZFY-10 marker indicated that Qaidam cattle owned Y1 and Y2 taurine haplogroups with frequencies of 0. 217 and 0.783, respectively. In Qaidam cattle breed, the frequencies of haplogroup Y2 in populations of Dachaidan, Wulan, Golmud, Mangya and Dulan were 1. 000, 0. 933, 0. 907, 0. 650 and 0. 522 respectively, while the frequencies of haplogroup Y1 were 0, 0. 067, 0. 093, 0. 350 and 0. 478 respectively, indicating that the populations of Mangya and Dulan had a higher proportion of Y1 haplogroup;3)The total haplotype diversity of Qaidam cattle was 0. 343 0±0. 045 6. In Qaidam cattle breed, the haplotype diversities in populations of Dachaidan, Wulan, Golmud, Mangya and Dulan were 0, 0. 133 3, 0. 172 8, 0. 478 9 and 0. 521 7, respectively. In conclusion, variations on Y chromosome ZFY-10marker could be used to determine the composition and origin of patrilineal branches in cattle. Qaidam cattle has two patrilineal branches with two paternal origins and had relatively lower paternal genetic diversity. Compared with Dachaidan, Wulan and Golmud populations, Dulan and Mangyapopulations had higher paternal genetic diversity in Qaidam cattle breed, showing that these two populations owned abundant genetic variations.关键词
柴达木黄牛/Y染色体/ZFY-10/单倍型组/遗传多样性/父系起源Key words
Qaidam cattle/Y chromosome/ZFY-10/haplogroup/genetic diversity/paternal origin分类
农业科技引用本文复制引用
马志杰,夏小婷,陈生梅,李瑞哲,张俸伟,晁生玉,雷初朝..基于Y染色体ZFY-10标记多态性探究柴达木黄牛父系支系组成和起源[J].中国农业大学学报,2018,23(6):70-76,7.基金项目
青海省自然科学基金项目(2017-ZJ-906) （2017-ZJ-906）
青海省"高端创新人才千人计划"项目 （）
国家自然科学基金项目(31360267) （31360267）
国家肉牛牦牛产业技术体系(CARS-37) （CARS-37）
青海省牛(奶、肉、绒)产业科技创新平台项目 （奶、肉、绒）
