中国输血杂志2018，Vol.31Issue(2)：113-116,4.DOI:10.13303/j.cjbt.issn.1004-549x.2018.02.005
5种ELISA试剂检测抗-HCV结果的差异分析
Difference analysis of the test results of hepatitis C antibody detection by 5 kinds of ELISA reagents
魏兰 1魏超 2李维 1王露楠 3尹丹 1张巧琳 1谢晓艳 1方建华 4朱海峰 5朱绍文 6释艳华7
作者信息
- 1. 重庆市血液中心,重庆 400015
- 2. 沧州市中心血站
- 3. 国家卫生计生委临床检验中心
- 4. 河南省红十字血液中心
- 5. 山东省血液中心
- 6. 江苏省血液中心
- 7. 襄阳市中心血站
- 折叠
摘要
Abstract
Objective To analyze the differences among the results by 5 kinds of ELISA reagents in hepatitis C antibody(anti-HCV) detection and to collect valuable data references for the blood screening reagent selection by blood banks. Methods The data of hepatitis C virus (HCV) was analyzed in the multi-center assessment project organized by the National Center for Clinical Laboratory of China. 5 reagents were selected for comparison and evaluation in sensitivity, specificity, the total coincidence rate, Youden index, accuracy and the false negative rate. Results A total of 1074 samples were tests with 242 positive and negative 832 cases. The results by 2 reagents in the five laboratories were highly consistent. There were significant differences between the two reagents in laboratory A, B, C, D (P＜0. 05), while no difference was found for reagent 5 and 4 in laboratory E (P＞0. 05) . There was no significant difference in the accuracy from the two reagents in laboratory B, C, D, E (P＞0. 05), while the accuracy of reagent 2 is better than reagent 1 in laboratory A (P＜0. 05) . There was no significant difference in the false negative rate of the two reagents in laboratory A, B, C, E (P＞0. 05), while the accuracy of reagent 5 is lower than reagent 2 in laboratory D (P＜0. 05) Conclusion The results of the two reagents in the 5 laboratories were highly consistent. Analysis of sensitivity, specificity, coincidence rate, Youden index, accuracy and the false negative rate showed that: Reagent 2 is superior to reagent 1 in laboratory A; Reagent 3 is superior to reagent 1 in laboratory B; Reagent 3 is superior to reagent 4 in laboratory C; Reagent 5 is superior to reagent 2 in laboratory D; Reagent 5 is superior to reagent4 in laboratory E.关键词
酶联免疫吸附法/抗-HCV/检测性能Key words
enzyme-linked immunosorbent assay/hepatitis C antibody/detection performance分类
医药卫生引用本文复制引用
魏兰,魏超,李维,王露楠,尹丹,张巧琳,谢晓艳,方建华,朱海峰,朱绍文,释艳华..5种ELISA试剂检测抗-HCV结果的差异分析[J].中国输血杂志,2018,31(2):113-116,4.
