中国输血杂志2018，Vol.31Issue(2)：117-120,4.DOI:10.13303/j.cjbt.issn.1004-549x.2018.02.006
重庆地区近2年血液核酸集中化检测结果分析
Result analysis of nucleic acid testing on blood samples in the Chongqing region with a macroscale collection-andtest detection model
谢晓艳 1王露楠 2张巧琳 1尹丹 1毕蕾静 1魏兰 1李维1
作者信息
- 1. 重庆市血液中心,重庆 400015
- 2. 国家卫生计生委临床检验中心
- 折叠
摘要
Abstract
Objcective To investigate the impact of nucleic acid testing(NAT) through systematic retrospective analysis on the nucleic acid test screening data of blood in Chongqing with a macroscale collection-and-test detection model. Methods Data from 1 city blood center and 14 district /county centers were collected and compared. All of the samples were analyzed by NAT and Enzyme-Linked Immuno Sorbent Assay(ELISA) in a parallel test setup. Moreover, nucleic acid identification test was conducted to identify NAT-positive samples. We analyzed the combined reaction rate、the NAT single reactive rate, the identification detection rate from data during the period between Jan, 2016 to Jun, 2017. We also followed up the HIVsuspected" window period"blood donors. Results A total of 20 9328 samples from Chongqing city Blood Center and 110 588 samples from the other 14 blood stations were tested by NAT. The combined reaction rate, the NAT single reactive rate, the identification detection rate were 0. 72％(1497 /20 9328) 、0. 34％(707 /20 9328) 、30. 98％(219 /707) in the Chongqing city Blood Center, respectively. These rates from the other 14 centers were 1. 1％(1228 /11 0588), 0. 61％(671 /11 0588), 36. 81％(247 /671), respectively. The nucleic acid identification test showed 212 HBV-DNA-reactive samples, 3 HIV-reactive samples, 0 HCV-reactive sample from Chongqing city Blood Center and 246 HBV-DNA-reactive samples, 1 HIV-reactive sample, 0 HCV-reactive sample from the other 14 blood stations. 4 reactive cases in the nucleic acid detection test were followed up, and 3 of them were confirmed as HIV"window period". There were regional differences in the rate of NAT single reactive and the rate of identification reactive analyzing the district /county data. Conclusion There are regional differences in the detection results in the districts and counties of Chongqing. Nucleic acid amplification technology presents high sensitivity and is capable of detecting the nucleic acid in the specimen and thus significantly reduces the"window period"of the virus, which ultimately reduce the risk of transmission of infectious diseases by blood.关键词
血液集中化检测/区域性差异/血液安全Key words
blood concentration test/regional difference/blood safety分类
医药卫生引用本文复制引用
谢晓艳,王露楠,张巧琳,尹丹,毕蕾静,魏兰,李维..重庆地区近2年血液核酸集中化检测结果分析[J].中国输血杂志,2018,31(2):117-120,4.
