中国输血杂志2018，Vol.31Issue(2)：147-151,5.DOI:10.13303/j.cjbt.issn.1004-549x.2018.02.014
四川骨髓库汉族人群HLA-DPB1与rs9277534等位基因频率及其连锁关系的分析
The frequency and linkage analysis of the HLA-DPB1 and rs9277534 alleles in the CMDP of Sichuan Han population
摘要
Abstract
Objective To investigate the distribution characteristics of HLA-DPB1 and rs9277534 alleles and their linkage linkage relationship in the China Marrow Donor Program (CMDP) of Sichuan Han population. Methods 200 samples were collected from unrelated healthy blood donors in the CMDP of Sichuan. The DNA of blood donors were extracted by blood DNA extraction kits. PCR-SBT was applied for HLA-DPB1 genotyping, and TaqMan was applied for rs9277534 genotyping. The Arlequin 3. 1 software was used for linkage disequilibrium analysis of the HLA-DPB1 and rs9277534 alleles . Results The genotype of DPB1* 05 ∶01: 01G allele was the most common with a frequency of 0. 412 5(165 /400) . The allele frequencies of A and G were 0. 4 (160 /400) and 0. 6 (240 /400) . AA, GG and AG genotype frequencies were 0. 165(33 /200), 0. 375(75 /200) and 0. 46(92 /200) . 10 HLA-DPB1 alleles were found to be in significant LD with the rs9277534 SNP. The DPB1* 04 ∶02 ∶01G, DPB1* 02 ∶01 ∶02G, DPB1* 02 ∶02 ∶01G and DPB1* 17 ∶01 ∶01G were in complete linkage with the rs9277534A allele, while DPB1* 05 ∶01 ∶01G, DPB1* 03 ∶01 ∶01G, DPB1* 13 ∶01 ∶01G, DPB1* 14 ∶01 ∶01G and DPB1* 21 ∶01, were in complete LD with the rs9277534G allele. Though not completely, DPB1* 04 ∶01 ∶01G(D' =0. 95; P＜0. 01) were found to be in significant LD with the rs9277534A allele. Conclusions This study expounds the LD between certain HLA-DPB1 and rs9277534A/G alleles in the CMDP of Sichuan Han Population, which provided valuable reference data for the clinical selection of donors in hematopoietic stem cell transplantation.关键词
HLA-DPB1 等位基因/rs9277534 等位基因/等位基因频率/连锁不平衡/移植物抗宿主病Key words
HLA-DPB1/rs9277534/allele frequency/LD/GVHD分类
医药卫生引用本文复制引用
于浩,王春艳,欧国进,纪欣,刘忠,王珏,陈强..四川骨髓库汉族人群HLA-DPB1与rs9277534等位基因频率及其连锁关系的分析[J].中国输血杂志,2018,31(2):147-151,5.基金项目
中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-024) （2016-I2M-3-024）
四川省科技厅项目资助(2017RZ0047) （2017RZ0047）
