中国兽医科学2018，Vol.48Issue(5)：559-565,7.DOI:10.16656/j.issn.1673-4696.2018.0106
重组猪α干扰素的纯化及其对猪繁殖与呼吸综合征病毒的抑制作用
Purification of recombinant porcine interferon-α and its inhibitory effect on porcine reproductive and respiratory syndrome virus
摘要
Abstract
The secretory expression of recombinant porcine interferon-α(rPoIFN-α) in Pichia pastoris was established. The highest concentration of rPoIFN-a reached 650.3 μg/mL at 84 h after initiating induction. The purity of rPoIFN-a from using affinity chromatography, ion exchange column chromatography and gel filtration column chromatography in turn was 95％ and the inhibitory activity of purified rPoIFN-a against vesicular stomatitis virus was about 5.63×106 IU/ mg using cytopathogenic effect inhibition assay.341 IU/mL rPoIFN-a pretreatment of Marc145 cells could completely inhibit the proliferation of porcine reproductive and respiratory syndrome virus (PRRSV). The treatment of PRRSV infected cells with 1367 IU/mL rPoIFN-a could completely inhibit the proliferation of PRRSV. The results indicated that rPoIFN-a has inhibitory effects on PRRSV infection in vitro, which provided scientific foundation for clinical application of rPoIFN-a.关键词
重组/猪α干扰素/纯化/猪繁殖与呼吸综合征病毒/抑制作用Key words
recombination/porcine interferon-α/purification/porcine reproductive and respiratory syndrome virus/inhibitory effect分类
农业科技引用本文复制引用
董世娟,于瑞嵩,李震,郑方方,谢春芳,陈冰清,姜平..重组猪α干扰素的纯化及其对猪繁殖与呼吸综合征病毒的抑制作用[J].中国兽医科学,2018,48(5):559-565,7.基金项目
国家自然科学基金项目(31602060) （31602060）
