中国兽医科学2018，Vol.48Issue(5)：586-592,7.DOI:10.16656/j.issn.1673-4696.2018.0090
保定地区奶牛源微小隐孢子虫子孢子表面抗原Cp23免疫特性的研究
Immunity characteristics of surface adhesion protein Cp23 on sporozoites and oocysts of Cryptosporidium parvum of dairy cow in Baoding
摘要
Abstract
To understand the immunity characteristics of sporozoite surface antigen Cp23 of Cryptosporidium parvum from dairy cow in Baoding of Hebei Province,4 weeks-old BALB/c mice were immunized with the Cp23 protein expressed from the recombinant plasmid pET28-Cp23. Then the humoral and cellular immunoresponses were evaluated. The results showed that the experiment group CD4+/CD8+T lymphocyte and the level of IgG were increased, and the mass concentration of IL-12 and IFN- γ were improved, there were significantly higher than those in the control groups (P<0.01). The recombinant protein Cp23 immunized mice could induce high humoral immunity and cellular immunity. The study provided material for the prevention of cryptosporidiosis in Baoding.关键词
微小隐孢子虫/Cp23重组蛋白/BALB/c小鼠/免疫特性Key words
Cryptosporidium parvum/recombinant protein Cp23/BALB/c mouse/immunity characteristic分类
农业科技引用本文复制引用
刘立元,康茜,任志军,王雪伟,唐欣浩,秦建华,赵月兰..保定地区奶牛源微小隐孢子虫子孢子表面抗原Cp23免疫特性的研究[J].中国兽医科学,2018,48(5):586-592,7.基金项目
河北省现代农业技术体系奶牛产业创新团队建设项目——奶牛疾病防控技术研发与示范(HBCT2013080204) （HBCT2013080204）
