中国兽医科学2018，Vol.48Issue(5)：593-604,12.DOI:10.16656/j.issn.1673-4696.2018.0114
猪红细胞免疫黏附功能与CR1-like表达水平的研究
Study on immune adhesion function and CRl-like expression level of porcine erythrocytes
摘要
Abstract
To provide theoretical data for subsequent research on the molecular mechanisms underlying the immune function of porcine erythrocyte CRl-like, in this study,we investigated the variability of porcine erythrocyte CRl-like at the individual level and determined its relationship with the immune adhesion of porcine erythrocytes. Flow cytometry was used to analyze the immune adhesion levels between Landrace porcine erythrocytes and fluorescent Escherichia coli and to determine the expression levels of erythrocyte CRl-like. One-way ANOVA,one-sample Kolmogorov-Smirnov test,and other statistical methods were used to compare the expression characteristics of erythrocyte CRl-like and its correlation with immune adhesion functions. Porcine erythrocyte immune adhesion could be classified into three types at the individual level:low,moderate,and high,with mean fluorescence values of 33.897± 3.294(34.483％ ),68.076 ±12.145 (46.552％ ), and 129.232±8.143(18.966％ ),respectively. CRl-like expression levels were also classified into three types at the individual level:low,moderate,and high, with mean fluorescence values of 21.479±0.865(14.583％ ),27.984±2.218(81.034％ ),and 36.690±0.058(6.897％ ),respectively. There was a significant correlation between erythrocyte adhesion levels and CRl-like expression levels (r=0.835,P<0.01),and regression analysis showed that both variables obey a classical S-shaped curve (lny=7.817 -102.911x,r2 =0.729,P< 0.01). In conclusion,variability in expression levels of erythrocyte CRl-like among Landrace porcine individuals are variable,which is correlated to porcine erythrocyte immune adhesion levels.关键词
猪/红细胞CR1-like/免疫黏附/表达量/差异性/相关性Key words
porcine/erythrocyte CRl-like/immune adhesion expression level/variability/correlation分类
农业科技引用本文复制引用
张静静,王春,贾瑞璞,尹伟,范阔海,孙娜,孙耀贵,李宏全..猪红细胞免疫黏附功能与CR1-like表达水平的研究[J].中国兽医科学,2018,48(5):593-604,12.基金项目
国家自然科学基金项目(31640082) （31640082）
山西省青年科技研究基金(201601D021110) （201601D021110）
