中国兽医科学2018，Vol.48Issue(5)：616-624,9.DOI:10.16656/j.issn.1673-4696.2018.0079
2006-2016年广西猪繁殖与呼吸综合征病毒流行毒株四个基因的进化动态分析
Evolutionary dynamics of Nsp2, ORF5, ORF6 and ORF7 genes of porcine reproductive and respiratory syndrome virus field strains in Guangxi Province from 2006 to 2016
摘要
Abstract
To investigate the molecular evolution of porcine reproductive and respiratory syndrome virus (PRRSV)field isolates,the Nsp2,ORF5,ORF6 and ORF7 genes were amplified from positive clinical samples in Guangxi province from 2006 to 2016,then were sequenced and analyzed. In result,the acquired Nsp2-1 and Nsp2-2 fragments are 1667 and 1649 bp in size,respectively,ORF5 gene is 603 bp in size,ORF6 gene is 525 bp in size,and ORF7 gene is 372 bp in size. The PRRSV field isolates all belonged to North American genotype PRRSV(NA-PRRSV). The homology identity of the nucleotide and deduced amino acid of Nsp2,ORF5,ORF6 and ORF7 genes were 86.8％ -100％ and 61.7％-100％, 80.8％-100％ and 80.5％-100％, 90.6％-100％ and 89.9％-100％, 85.6％-100％ and 87.1％-100％, respectively. The phylogenetic analysis showed that all the PRRSV isolates were divided into six subgroups based on Nsp2,ORF5,ORF6 and ORF7 genes,and most of the isolates from Guangxi Province belonged to subgroup 6,which was represented by JXA1 strain. The average substitution rates of Nsp2,ORF5,ORF6 and ORF7 genes of PRRSV isolates were 1.69×10-1,1.58×10-2,9.71×10-4 and 1.86 × 10-3 substitutions/site/year respectively, indicating that Nsp2 gene is the most easily mutated,followed by ORF5 gene,then by ORF7 gene,and ORF6 gene was the most stable among the four genes. The results of this study provided fundamental data for PRRSV molecular epidemiology and evolution.关键词
猪繁殖与呼吸综合征病毒/基因/遗传变异/进化动态Key words
porcine reproductive and respiratory syndrome virus (PRRSV)/gene/genetic variation/evolutionary dynamics分类
农业科技引用本文复制引用
尹彦文,施开创,王海清,莫胜兰,屈素洁..2006-2016年广西猪繁殖与呼吸综合征病毒流行毒株四个基因的进化动态分析[J].中国兽医科学,2018,48(5):616-624,9.基金项目
广西科学研究与技术开发计划项目(桂科转14125004-22) （桂科转14125004-22）
广西科技重大专项(桂科AA17204057) （桂科AA17204057）
